Please note: This alignment was not rigorously constructed! The alignment was done by manually aligning conserved regions using MSI's Insight/Homology program. The alignment is most reliable within the EF-hands. The alignment of the regions outside of the EF-hands is untrustworthy, particularly in the loop linking the two EF-hands in a domain. A more rigorous alignment is planned for the future.
Kawaski and Kretsinger's alignment from Protein Profile,
1 was used to aid in aligning many of these sequences.
S100 family alignment is from Potts, et al.
Nature Struct. Biol. (1995) 2. 790-796.
The summary page gives the identifying letters for each protein.
Click on the name of a protein to go to that protein's "homepage."
Click on a residue in the consensus sequence to see information about that
particular residue, including information about mutations made at that site in
various proteins.
|-helixI--|---loop1------|helixII|
En**nn**-nX**Y-*Z*G#Ix**zn**nn**n
S100 family
CALB9 :1 KSPEELKGIFEK-YAAKE-GDPNQLSKEELKLLLQTE 35
CALCYC :1 MASPLDQAIGLLIGIFHK-YSGKE-GDKHTLSKKELKELIQKE 41
MRP-8 :1 MLTELEKALNSIIDVYHK-YSLIK-GNFHAVYRDDLKKLLETE 41
MRP-14 :1 MTCKMSQLERNIETIINTFHQ-YSVKL-GHPDTLNQGEFKELVRKD 44
CALGRAN-C :1 TKLEDHLEGIINIFHQ-YSVRL-GHYDTLIKRELKQLITKE 39
p11 :1 PSQMEHAMETMMFTFHK-FAGDK-GY---LTKEDLRVLMEKE 37
S100A :1 GSELETAMETLINVFHA-HSGKE-GDKYKLSKKELKELLQTE 40
S100B :1 SELEKAVVALIDVFHQ-YSGRE-GDKHKLKKSELKELINNE 39
S100C :1 MAKRPTETERCIESLIAIFQK-HAGRD-GNNTKISKTEFLIFMNTE 44
S100D :1 MPAAWILWAHSHSELHTVMETPLEKALTTMVTTFHK-YSGRE-GSKLTLSRKELKELIKKE 59
S100E :1 MARPLEQAVAAIVCTFQE-YAGRC-GDKYKLCQAELKELLQKE 41
S100L :1 MSSPLEQALAVMVATFHK-YSGQE-GDKFKLSKGEMKELLHKE 41
S100P :1 MTELETAMGMIIDVFSR-YSGSE-GSTQTLTKGELKVLMEKE 40
CAPL :1 MACPLEKALDVMVSTFHK-YSGKE-GDKFKLNKSELKELLTRE 41
CaM-Like
CALMOD-I :1 ADQLTEEQIAEFKEAFSL-FD-KD-GD-GTITTKELGTVMRSL 39
CALMOD-II :76 MKDTDSEEEIREAFRV-FD-KD-GN-GYISAAELRHVMTNL 112
CALTRCT-I :1 MSYKAKTVVSARRDQKKGRVGLTEEQKQEIREAFDL-FD-TD-GS-GTIDAKELKVAMRAL 57
CALTRCT-II :95 MGERDSREEILKAFRL-FD-DD-NS-GTITIKDLRRVAKEL 132
TROPC-I :1 ASMTDQQAEARAFLSEEMIAEFKAAFDM-FD-AD-GG-GDISTKELGTVMRML 49
TROPC-II :86 MKEDAKGKSEEELANCFRI-FD-KN-AD-GFIDIEELGEILRAT 125
CALL-I :1 ADQLTEEQVTEFKEAFSL-FD-KD-GD-GCITTRELGTVMRSL 39
CALL-II :76 MKDTDNEEEIREAFRV-FD-KD-GN-GFVSAAELRHVMTRL 112
CDPK-I :1(329) ....AERLSEEEIGGLKELFKM-ID-TD-NS-GTITFDELKDGLKRG 39(367)
CDPK-II :75(403) NKLEREENLVSAFSY-FD-KD-GS-GYITLDEIQQACKDF 110(438)
SQUID-I :1 AKELSEKQIAEIKDAFDM-FD-ID-GD-GQITSKELRSVMKSL 39
SQUID-II :76 MGPTDPEKEMREAFRV-FD-KD-GN-GLITAAELRQVMANF 112
Myosin Light Chains
MYO_ELC-I :1 PKLSQDEIDDLKDVFEL-FDFWD-GRDGAVDAFKLGDVCRCL 40
MYO_ELC-II :76 MDCEQGTFADYMEAFKT-FD-RE-GQ-GFISGAELRHVLTAL 113
MYO_RLC-I :1 ADKAASGVLTKLPQKQIQEMKEAFSM-ID-VD-RD-GFVSKEDIKAISEQL 47
MYO_RLC-II :80 LSGTDSEETIRNAFAM-FD-EQ-ET-KKLNIEYIKDLLENM 116
Parvalbumin
PARV :1 AKDLLKADDIKKALDAVKAEGSFNHKKFFALVGLKAMSANDVKKVFKA-ID-AD-AS-GFIEEEELKFVLKSF 69
Spectrin
SPECTRIN :1(2320) ....RNTTGVAEEALKEFSMM-FKHFD-KD-KS-GRLDHQEFKSCLRSL 41(2360)
Calcineurin
CALCIN-I :1 GNEASYPLEMCSHFDADEIKRLGKRFKK-LD-LD-NS-GSLSVEEFMSLP-EL 48
CALCIN-II :82 SVKGDK-EQKLRFAFRI-YD-MD-KD-GYISNGELFQVLKMM 118
Calbindin D28k, calretinin
CALB28-I :1 AESHLQSSLITASQFFEIWLH-FD-AD-GS-GYLEGKELQNLIQEL 42
CALB28-II :92 RCQQLKSCEEFMKTWRK-YD-TD-HS-GFIETEELKNFLKDL 130
CALB28-III :187 CGKEFNKAFEL-YD-QD-GN-GYIDENELDALLKDL 219
CALRET-I :1 MAGPQQQPPYLHLAELTASQFLEIWKH-FD-AD-GN-GYIEGKELENFFQEL 48
CALRET-II :95 FLLCFRQHVGSSAEFMEAWRK-YD-TD-RS-GYIEANELKGFLSDL 137
CALRET-III :195 TSEEFNAIFTF-YD-KD-RS-GYIDEHELDALLKDL 227
Neuronal Calcium Sensors
HIPP-I :1 GKQNSKLRPEMLQDLRENTEFSELELQEWYKGF-LK-DC-PT-GILNVDEFKKIYANF 54
HIPP-II :97 LEQKLMWAFSM-YD-LD-GN-GYISREEMLEIVQAI 129
RECOV-I :2 GNSKSGALSKEILEELQLNTKFTEEELSSWYQSF-LK-EC-PS-GRITRQEFQTIYSKF 56
RECOV-II :98 TNQKLEWAFSL-YD-VD-GN-GTISKNEVLEIVTAI 130
Calpain
CANPI-I :1(541) ....EEIDENFKALFR-QL-AG-ED-MEISVKELRTILNRI 33(573)
CANPI-II :77(617) IRNYLSIFRK-FD-LD-KS-GSMSAYEMRMAIESA 107(647)
CANPI-III :142(682) LETMFRFFKT-LD-TD-LD-GVVTFDLFKWLQLTMFA 174(715)
CANPII-I :1(531) ....DDGVRRLFA-QL-AG-ED-AEISAFELQTILRRV 30(560)
CANPII-II :74(604) IQKYQKIYRE-ID-VD-RS-GTMNSYEMRKALEEA 103(633)
CANPII-III :138(668) LETLFKIFKQ-LD-PE-NT-GTIELDLISWLCFSVL 169(699)
CANPIII-I :1(651) ....QQQFRNIFK-QI-AG-DD-MEICADELKKVLNTV 30(680)
CANPIII-II :74(724) IKAWQKIFKH-YD-TD-QS-GTINSYEMRNAVNDAA 103(653)
CANPIII-III:138(788) LEGMFRAFHA-FD-KD-GD-GIIKLNVLEWLQLTMYA 169(819)
smCANP-I :1(91) ....EANESEEVRQFRRLFA-QL-AG-DD-MEVSATELMNILNKV 37(127)
smCANP-II :81(171) IKRWQAIYKQ-FD-TD-RS-GTICSSELPGAFEAA 111(201)
smCANP-III :146(236) LDAMFRAFKS-LD-KD-GT-GQIQVNIQEWLQLTMYS 178(268)
Sarcoplasmic CaBP
AMP_SARC-I :1 GLNDFQKQKIKFTFDFFLD-MN-HD-GSIQDNDFEDMMTRY 38
AMP_SARC-II:90 IATCKSVADLP---AWCQNRIPFLFKG-MD-VS-GD-GIVDLEEFQNYCKNF 134
NER_SARC-I :1 SDLWVQKMKTYFNR-ID-FD-KD-GAITRMDFESMAERF 35
NER_SARC-II:80 ----KEMVKNPEAKSVVEGPLPLFFRA-VD-TN-ED-NNISRDEYGIFFGML 123
Others
BM40 :1(205) ......KNYNMYIFPVHWQFGQ-LD-QHPID-GYLSHTELAPLRAPL 38(238)
|---linker loop-------|helixIII|-|--loop2----|helixIV|
-* En**nn**n--X*-Y*ZG#Ix**zn**nn**n*
S100 family
CALB9 :36 -FPSLLKGGS-------------TLDELFEEL--DK-NGDGEVSFEEFQVLVKKISQ 75
CALCYC :42 -LTIGSKLQDA------------EIVKLMDDL--DR-NKDQEVNFQEYITFLGALAMIYNEALKG 90
MRP-8 :42 -CPQYIRKK--------------GADVWFKEL--DI-NTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE 93
MRP-14 :45 -LQNFLKKENKNEK---------VIEHIMEDL--DT-NADKQLSFEEFIMLMARLTWASHEKMHE-GDEGPGHHHKPGLGEGTP 114
CALGRAN-C :40 -LPNTLKN-TKDQG---------TIDKIFQNL--DA-NQDEQVSFKEFVVLVTDVLITAHDNIHKE 91
p11 :38 -FPGFL-ENQPL-----------AVDKIMKDL--DQ-CRDGKVGFQSFFSLIAGLTIACNDYFVVHMKQKGK 95
S100A :41 -LSGFl-DAQKDAD---------AVDKVMKEL--DE-NGDGEVDFQEYVVLVAALTVACNNFFWENS 93
S100B :40 -LSHFL-EEIKEQE---------VVDKVMETL--DS-DGDGECDFQEFMAFVAMITTACHEFFEHE 91
S100C :45 -LAAFT-QNQKDPG---------VLDRMMKKL--DL-DSDGQLDFQEFLNLIGGLAIACHDSFIKSTQK 99
S100D :60 -L--CL-GE-MKES---------SIDDLMKSL--DK-NSDQEIDFKEYSVFLTMLCMAYNDFFLEDNK 110
S100E :42 -LATWT-PTEFREC---------DYNKFMSVL--DT-NKDCEVDFVEYVRSLACLCLYCHEYFKDCPSEPPCSQ 101
S100L :42 -LPSFV-GEKVDEE---------GLKKLMGDL--DE-NSDQQVDFQEYAVFLALITIMCNDFFQGSPARS 97
S100P :41 -LPGFL-QSGKDKD---------AVDKLLKDL--DA-NGDAQVDFSEFIVFVAAITSACHKYFEKAGLK 95
CAPL :42 -LPSFL-GKRTDEA---------AFQKLMSNL--DS-NRDNEVDFQEYCVFLSCIAMMCNEFFEGFPDKQPRKK 101
CaM-Like
CALMOD-I :40 -GQNPTEA---------------ELQDMINEV--DA-DGNGTIDFPEFLTMMARK 75
CALMOD-II :113 -GEKLTDE---------------EVDEMIREA--DI-DGDGQVNYEEFVQMMTAK 148
CALTRCT-I :58 -GFEPKKE---------------EIKKMISEI--DK-DGSGTIDFEEFLTMMTAK 94
CALTRCT-II :133 -GENLTEE---------------ELQEMIAEA--DR-NDDNEIDEDEFIRIMKKTSLF 172
TROPC-I :50 -GQNPTKE---------------ELDAIIEEV--DE-DGSGTIDFEEFLVMMVRQ 85
TROPC-II :126 -GEHVTEE---------------DIEDLMKDS--DK-NNDGRIDFDEFLKMMEGVQ 162
CALL-I :40 -GQNPTEA---------------ELRDMMSEI--DR-DGNGTVDFPEFLGMMARK 75
CALL-II :113 -GEKLSDE---------------EVDEMIRAA--DT-DGDGQVNYEEFVRVLVSK 148
CDPK-I :40 -SELMES----------------EIKDLMDAA--DI-DKSGTIDYGEFIAATVHL 74(402)
CDPK-II :111 -GLDDI-----------------HIDDMIKEI--DQ-DNDGQIDYGEFAAMMRKGNGGIGRRTMRK 155(483)
SQUID-I :40 -GRTPSDA---------------ELEEMIREV--DT-DGNGTIEYAEFVEMMAKQ 75
SQUID-II :113 -SDEKLTSE--------------EISEMIREA--DI-DGDGMVNYEEFVKMMTPK 149
Myosin Light Chains
MYO_ELC-I :41 -GINPRN----------------EDVFAVGGT--HK-MGEKSLPFEEFLPAYEGL 75
MYO_ELC-II :114 -GERLSDE---------------DVDEIIKLTDLQE-DLEGNVKYEDFVKKVMAGPYPDK 156
MYO_RLC-I :48 -GRAPDDK---------------ELTAML-----KE-APGPLNFTMFLSIFSDK 79
MYO_RLC-II :117 -GDNFNKD---------------EMRMTFKEA--PV-E-GGKFDYVKFTAMIKGSGEEEA 156
Parvalbumin
PARV :70 -AADGRDLTDA------------ETKAFLKAA--DK-DGDGKIGIDEFETLVHEA 108
Spectrin
SPECTRIN :42 -GYDLPMVEEGEPDP--------EFESILDTV--DP-NRDGHVSLQEYMAFMISR 84(2403)
Calcineurin
CALCIN-I :49 -QQNP------------------LVQRVIDIF--DT-DGNGEVDFKEFIEGVSQF 81
CALCIN-II :119 VGNNLKDTQLQQ-----------IVDKTIINA--DK-DGDGRISFEEFCAVVGGLDIHKKMVVDV 169
Calbindin D28k, calretinin
CALB28-I :43 -QQARKKAGLELSP---------EMKTFVDQY--GQ-RDDGKIGIVELAHVLPTEENFLLLF 91
CALB28-II :130 -LEKANKTVDDTKLAE-------YTDLMLKLF--DS-NNDGKLELTEMARLLPVQENFLLKFQGIKM 186
CALB28-III :220 -CEKNKQDLDINN----------ITTYKKNIM--AL-SDGGKLYRTDLALILCAGDN 253
CALRET-I :49 -EKARKGSGMMSKSDNFGE----KMKEFMQKY--DK-NSDGKIEMAELAQILPTEEN 94
CALRET-II :138 -LKKANRPYDEPKLQE-------YTQTILRMF--DL-NGDGKLGLSEMSRLLPVQENFLLKFQGMKL 194
CALRET-III :228 -YEKNKKEINIQQLT--------NYRKSVMSL--AE-AGKLYRKDLEIVLCSEPPM 272
Neuronal Calcium Sensors
HIPP-I :55 -FPYGDASK--------------FAEHVFRTF--DT-NSDGTIDFREFIIALSVTSPGR 96
HIPP-II :130 -YKMVSS---VMKMPEDESTPEKRTEKIFRQM--DT-NNDGKLSLEEFIRGAKSDPSIVRLLQCDPSSRSQF 195
RECOV-I :57 -FP--------------EADPKAYAQHVFRSF--DA-NSDGTLDFKEYVIALHMTSAGK 97
RECOV-II :131 -FKMISPEDTKHLPEDENTP---EKRAEKIWG--FF-GKKDDDKLTEKEFIEGTLANKEILRLIQFEPQKVKEKLKEKKL 204
Calpain
CANPI-I :34 -ISKHKDLRTKGFSLE-------SCRSMVNLM--DR-DGNGKLGLVEFNILWNR 76(616)
CANPI-II :108 -GFKLNK----------------KLYELIITR--YS-EPDLAVDFDNFVCCLVR 141(681)
CANPII-I :31 -LAKRQDIKSDGFSIE-------TCKIMVDML--DS-DGSGKLGLKEFYILWTK 73(603)
CANPII-II :104 -GFKMPC----------------QLHQVIVAR--FA-DDQLIIDFDNFVRCLVR 137(667)
CANPIII-I :31 -VNKHKDLKTHGFTLE-------SCRSMIALM--DT-DGSGKLNLQEFHHLWNK 73(723)
CANPIII-II :104 -GFHLNN----------------QLYDIITMR--YA-DKHMNIDFDSFICCFVR 137(787)
smCANP-I :38 -VTRHPDLKTDGFGID-------TCRSMVAVM--DS-DTTGKLGFEEFKYLWNN 80(170)
smCANP-II :112 -GFHLNE----------------HLYNMIIRR--YS-DESGNMDFDNFISCLVR 145(235)
Sarcoplasmic CaBP
AMP_SARC-I :39 -KEVNKGSLSDADYKSMQASLEDEWRDLKGRA--DI-NKDDVVSWEEYLAMWEKT 89
AMP_SARC-II:135 -QLQCADVP--------------AVYNVITDG--GKVTFDLNRYKELYYRLLTSPAADAGNTLMGQKP 185
NER_SARC-I :36 ----AKE---SEMKAEHAKVLMDSLTGVWDNF---L-TAVAGGKGIDETTFINSM 79
NER_SARC-II:124 -GLDKT-----------------MAPASFDAI--DT-NNDGLLSLEEFVIAGSDF--FM-NDGDSTNKVFWGPLV 174
Others
BM40 :39 -IPMEH-----------------CTTRFFETC--DL-DNDKYIALDEWAGCFGIKQKDIDKDLVI 82(286)
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