Home Page for Calbindin D28k

Calbindin D28k

Primary information about calbindin D28k:
Basic chemical and physical information	Basic biological information	Basic genomic information	Metal ion binding constants	Interspecies sequence alignment	Information on function
Summary of structural studies	Information on the conformational change	List of available structures	Information about mutations	References about calbindin D28k	Other web resources about calbindin D28k
Additional information about calbindin D28k:
Information about the evolutionary relationship of calbindin D28k to other proteins

Basic Information

Basic Chemical and Physical Information:
Additional names and abbreviations:	calbindin D28k: vitamin D-dependent calcium-binding protein
Isoforms:	No information about isoforms is stored in the database
Yeast Protein Database Entries:	No links to the YPD are stored in the database

Number of amino acids:	260 (Bos taurus, Rattus norvegicus, Mus musculus, Homo sapiens) - 261 (Gallus gallus) View a list of all sources stored in the database
Molecular weight:	29.384 kD (Bos taurus) - 30.036 kD (Gallus gallus) View a list of all sources stored in the database
pI:	not yet available in the data library
Number of functional calcium binding sites:	not yet available in the data library
Macroscopic calcium binding constants:	not yet available in the data library
Other metal binding constants:	not yet available in the data library
Protein stability:	not yet available in the data library
Post-translational modifications:	not yet available in the data library

Basic Biological Information:
Source organisms:	Bos taurus, Gallus gallus, Homo sapiens, Mus musculus, Rattus norvegicus View an annotated list of all sources stored in the database
Tissues:	birds: intestine (InfoCard) mammals: brain (InfoCard) mammals: kidney (InfoCard) mammals: pancreas (InfoCard) Gallus gallus: egg shell gland (uterus) (InfoCard)
Subcellular localization:	not yet available in the data library
Regulation:	all sources: regulated by Vitamin D (upregulates expression) (InfoCard)

Genomic Information:
GenBank entries:	View a list of GenBank entries stored in the database
Gene length:	not yet available in the data library
Gene structure:	not yet available in the data library
Promoter:	not yet available in the data library
Gene copies:	not yet available in the data library
Alleles:	no alleles are currently stored in the data library
Chromosomal localization:	Homo sapiens, expressed (GenBank code X06661): q21.3-2 region of chromosome 8 (InfoCard)

Interspecies Sequence Alignment



                     |-helixI-|--loop1----|helixII-|
                     En**nn**nX*Y*ZG#Ix**zn**nn**n*

MOUSE  : -AESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELL
RAT    : -AESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELL
HUMAN  : -AESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQ
BOVINE : -AESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQ
CHICK  : TAETHLQGVEISAAQFFEIWHHYDSDGNGYMDGKELQNFIQELQ

                     |helixIII|--loop2----|helixIV|
                     En**nn**nX*Y*ZG#Ix**zn**nn**n*

MOUSE  : QARKKAGLELSPEMKSFVDQYGQRDDGKIGIVELAHVLPTEENF
RAT    : QARKKAGLELSPEMKTFVDQYGQRDDGKIGIVELAHVLPTEENF
HUMAN  : QARKKAGLELSPEMKTFVDQYGQRDDGKIGIVELAHVLPTEENF
BOVINE : QARKKAGLELSPEMKTFVDQYGERDDGKIGIVELAHVLPTEENF
CHICK  : QARKKAGLDLTPEMKAFVDQYGKATDGKIGIVELAQVLPTEENF

                       |helixV-|-loop3-----|helixVI-|
                       En**nn**nX*Y*ZG#Ix**zn**nn**n*

MOUSE  : LLLFRC-QQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAE
RAT    : LLLFRC-QQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAE
HUMAN  : LLLFRC-QQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAE
BOVINE : LLLFRC-QQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAE
CHICK  : LLFFRC-QQLKSSEDFMQTWRKYDSDHSGFIDSEELKSFLKDLLQKANKQIEDSKLTE

        |helixVII|--loop4-----|hlxVIII|
         En**nn**nX*Y*ZG#Ix**zn**nn**n*              

MOUSE  : YTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGK
RAT    : YTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGK
HUMAN  : YTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGK
BOVINE : YTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGVKMCGK
CHICK  : YTEIMLRMFDANNDGKLELTELARLLPVQENFLIKFQGVKMCAK

         |hlxIX--|--loop5----|-helixX-|
         En**nn**nX*Y*ZG#Ix**zn**nn**n*

MOUSE  : EFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQELDINN
RAT    : NGYIDENELDALLKDLCEKNKQELDINNEFNKAFELYDQDG
HUMAN  : NGYIDENELDALLKDLCEKNKQDLDINNEFNKAFELYDQDG
BOVINE : DGYIDENELDALLKDLCEKNKQDLDINNEFNKAFELYDQDG
CHICK  : NGYIDENELDALLKDLCEKNKKELDINNEFNKAFEMYDQDG

         |hlxXI--|--loop6----|helixXII|
         En**nn**nX*Y*ZG#Ix**zn**nn**n*

MOUSE  : ITTYKKNIMALSDGGKLYRTDLALILSAGDN
RAT    : ISTYKKNIMALSDGGKLYRTDLALILSAGDN
HUMAN  : ITTYKKNIMALSDGGKLYRTDLALILCAGDN
BOVINE : IPTYKKSIMALSDGGKLYRTDLALILSAGDN
CHICK  : LATYKKSIMALSDGGKLYRAELALILCAEEN

Sequence alignment for entire calbindin D28k/calretinin subfamily

Functional Information:

General information:

no general functional information is currently stored in the database

Biological roles:

calcium transport and uptake in avian intestine
biochemical evidence in Gallus gallus: calbindin D9k was found to facilitate diffusion of calcium in a flow-dialysis cell at near physiological calcium concentrations (InfoCard)
calcification of bone
immunocytochemical or immunohistochemical evidence in Gallus gallus: calbindin D28k is found in the correct locations to support activity in calcification of bone (InfoCard)
calcification of teeth
immunocytochemical or immunohistochemical evidence in Rattus norvegicus: calbindin D28k is found in the correct locations to support activity in calcification of teeth (InfoCard)

Disease states:

epilepsy
model system evidence : There is a specific decline in calbindin D28k in the dentate granule cells of the hippocampus during the process of kindling mice to establish the kindling model for epilepsy (InfoCard)
amyotrophic lateral sclerosis
immunocytochemical or immunohistochemical evidence : Calbindin D28k immunoreactivity is specifically absent from neuron poplulations lost early in ALS (InfoCard)
Huntington's disease
immunocytochemical or immunohistochemical evidence : Immunohistochemical studies showed a substantial loss of neurons containing calbindin D28k in autopsy specimens from patients who died with a diagnosis of Huntington's disease (InfoCard)

Target molecules:

not yet available in the data library

Structural Information:

Calcium-coordination:
not yet available in the data library

Nature of the conformational changes:
not yet available in the data library

Secondary structure: (as reported in the PDB file and its accompanying reference)
not yet available in the data library

Additional structural information:
no additional structural information is currently stored in the database

Available structures: (follow the links from the PDB code to the retrieve the PDB files)
no structures are currently stored in the database

Information about mutants:

no mutants are currently stored in the database