Non-Commercial Vectors

CSB plasmids are freely available to any Vanderbilt researcher. Contact Laura Mizoue for more information. The links below are restricted to access from Vanderbilt only.

Prokaryotic Expression:

NT Tag(s)	Protease Cleavage Site	CT Tag(s)	Resistance	Vector
GST	TEV	His8	Kan	pAT110
GST	3C/PreScission	His8	Kan	pAT109
His6	Thrombin	HSV•His6	Kan	pSV277
	TEV	HSV•His6	Kan	pSV281
	3C/PreScission	HSV•His6	Kan	pBG100
His10	Thrombin	His8	Kan	pNR114
	TEV	His8	Kan	pNR111
	3C/PreScission	His8	Kan	pBG106
His•GST	TEV	HSV•His6	Kan	pBG105
His•GST	3C/PreScission	HSV•His6	Kan	pBG101
His•MBP	Thrombin	HSV•His6	Kan	pSV278
	TEV	HSV•His6	Kan	pSV282
	3C/PreScission	HSV•His6	Kan	pLM302 pLM303
His•SUMO	3C/PreScission	HSV•His6	Kan	pBG102 pBG103
His•SUMO	Ulp1	HSV•His6	Kan	pBG104
MBP	Tev	His8	Kan	pAT108
MBP	3C/PreScission	His8	Kan	pAT107
Trx•His	Tev	His6	Amp	pNR113
	3C/PreScission	HSV•His6	Kan	pLM304
	3C/PreScission	His6	Amp	pNR112

Recombinant Protease Expression:

TEV protease
3C protease
Ulp1