pH6H3C
For expression of N-terminally His-tagged human rhinovirus 3C protease in E. coli.
General Information:
- pET derivative
- T7lac promoter
- low copy
- Kan resistance
-
dna sequence
- 3C protease recognition sequence: LEVLFQ↓GP
- MW = 21.8 kDa
- Amino acid sequence:
MGSSHHHHHH GGGLVPRGPN TEFALSLLRK NIMTITTSKG EFTGLGIHDR VCVIPTHAQP
GDDVLVNGQK IRVKDKYKLV DPENINLELT VLTLDRNEKF RDIRGFISED LEGVDATLVV
HSNNFTNTIL EVGPVTMAGL INLSSTPTNR MIRYDYATKT GQCGGVLCAT GKIFGIHVGG
NGRQGFSAQL KKQYFVEKQ
Protocol: 3C protease expression and purification This document also contains some information on buffer components that affect protease activity.
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