pH6H3C

For expression of N-terminally His-tagged human rhinovirus 3C protease in E. coli.

General Information:

pET derivative

T7lac promoter

low copy

Kan resistance

dna sequence

3C protease recognition sequence: LEVLFQ↓GP

MW = 21.8 kDa

Amino acid sequence:

MGSSHHHHHH GGGLVPRGPN TEFALSLLRK NIMTITTSKG EFTGLGIHDR VCVIPTHAQP GDDVLVNGQK IRVKDKYKLV DPENINLELT VLTLDRNEKF RDIRGFISED LEGVDATLVV HSNNFTNTIL EVGPVTMAGL INLSSTPTNR MIRYDYATKT GQCGGVLCAT GKIFGIHVGG NGRQGFSAQL KKQYFVEKQ

Protocol: 3C protease expression and purification
This document also contains some information on buffer components that affect protease activity.