Duet Expression Vectors
All vectors contain 2 MCS each preceded by a T7lac promoter and rbs. Vectors can be used in combination to express more than 2 proteins. See the Novagen web site for further details.
Vectors:
Vector |
MCS1 Tag |
MCS2 Tag |
Resistance* |
Replicon |
Sequence |
pCDFDuet-1 |
NT His6 |
None |
Strep/Spect |
CloDF13 |
genbank map |
pCOLADuet-1 |
NT His6 |
None |
Kan |
ColA |
genbank map |
pETDuet-1 |
NT His6 |
None |
Amp |
ColE1 |
genbank map |
pETDuetMOD1 |
NT His6, Tev cleavable |
None |
Amp |
ColE1 |
genbank |
pETDuetMOD2 |
NT His6 |
NT His6, 3C cleavable |
Amp |
ColE1 |
genbank |
pETDuetMOD3 |
NT His6, 3C cleavable |
None |
Amp |
ColE1 |
genbank |
pRSFDuet-1 |
NT His6 |
None |
Kan |
RSF1030 |
genbank map |
pRSFDuetMOD1 |
NT His6, 3C cleavable |
None |
Kan |
RSF1030 |
genbank |
*Amp=ampicillin; Kan=kanamycin; Spect=spectinomycin; Strep=streptomycin
Sequencing Primers:
You cannot use the T7 universal forward primer for sequencing Duet vectors.
Name |
Use |
Sequence |
Duetseq |
Forward, 1st MCS, pETDuet (Binds further upstream than pET Upstream) |
5'-GGTGCATGCAAGGAGATGG |
DuetDOWN1 |
Reverse, 1st MCS, all Duet vectors |
5'-GATTATGCGGCCGTGTACAA |
DuetUP2 |
Forward, 2nd MCS, all Duet vectors |
5'-TTGTACACGGCCGCATAATC |
pColaUP |
Forward, 1st MCS, pCDFDuet, pCOLADuet, pRSFDuet (Identical to ACYCDuetUP1) |
5'-GGATCTCGACGCTCTCCCT |
pET Upstream |
Forward, 1st MCS, pETDuet |
5'-ATGCGTCCGGCGTAGA |
T7Rev |
Reverse, 2nd MCS, all Duet vectors |
5'-GCTAGTTATTGCTCAGCGGT |
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