pH6Ulp1

For expression of N-terminally His-tagged Ulp1 (Sumo protease) in E. coli.

General Information:

  • pET derivative
  • T7lac promoter
  • low copy
  • Kan resistance
  • dna sequence
  • Encodes catalytic domain of S. cerevisiae Ulp1, residues 430-621
  • MW = 26.8 kDa
  • Amino acid sequence:
MGSSHHHHHH GGGLVPELNE KDDDQVQKAL ASRENTQLMN RDNIEITVRD FKTLAPRRWL NDTIIEFFMK YIEKSTPNTV AFNSFFYTNL SERGYQGVRR WMKRKKTQID KLDKIFTPIN LNQSHWALGI IDLKKKTIGY VDSLSNGPNA MSFAILTDLQ KYVMEESKHT IGEDFDLIHL DCPQQPNGYD CGIYVCMNTL YGSADAPLDF DYKDAIRMRR FIAHLILTDA LK

Protocol: Ulp1 expression and purification