pBG104
- N-terminal 6xHis + SUMO tag; Ulp1 cleavable
- Optional C-terminal HSV+6xHis tags; non-cleavable
- pET27 derivative; Sumo tag from pSUMO
- T7lac promoter
- Kan resistant
- 5' cloning site: BsaI
- low copy
-
dna sequence
- Tag cleavage with Ulp1 produces native protein
- Cleavage rates with Ulp1 may vary depending on the identity of the first residue of the protein of interest
- Ulp1 will not cleave if the first residue is a proline
- MW of 6xHis + SUMO tag = 12.7 kDa
- graphic map (Digestion with BsaI produces a sticky end as indicated by the purple triangles):
More information on how to clone into pBG104 can be downloaded here as a pdf file.
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