pLM303

• N-terminal 6xHis + Maltose Binding Protein (MBP) tag; 3C protease cleavable
• Optional C-terminal HSV+6xHis tags; non-cleavable
• pET27 derivative; MBP tag from pMAL
• T7lac promoter
• Kan resistant
• Similar to pLM302 but with different 5' cloning site: KpnI
• KpnI site overlaps coding region of 3C protease recognition sequence. You must add an extra nucleotide to the start of your gene for it to be in frame.
• Low copy
• dna sequence
• MW of His+MBP tag ~ 44 kDa
• graphic map: