pLM303
- N-terminal 6xHis + Maltose Binding Protein (MBP) tag; 3C protease cleavable
- Optional C-terminal HSV+6xHis tags; non-cleavable
- pET27 derivative; MBP tag from pMAL
- T7lac promoter
- Kan resistant
- Similar to pLM302 but with different 5' cloning site: KpnI
- KpnI site overlaps coding region of 3C protease recognition sequence. You must add an extra nucleotide to the start of your gene for it to be in frame.
- Low copy
-
dna sequence
- MW of His+MBP tag ~ 44 kDa
- graphic map:
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