pBG103

• N-terminal 6xHis + SUMO tag; 3C protease cleavable
• Optional C-terminal HSV+6xHis tags; non-cleavable
• pET27 derivative; Sumo tag from pSUMO
• T7lac promoter
• Kan resistant
• 5' cloning site: KpnI
• Note: KpnI site overlaps coding region of 3C protease recognition sequence. You must add an extra nucleotide to the start of your gene for it to be in frame.
• Low copy
• dna sequence
• MW of His+SUMO tag ~13.6 kDa
• graphic map: