pAT109

• N-terminal GST tag; 3C protease cleavable
• Optional C-terminal 8xHis tag; non-cleavable
• Note: This vector has a KpnI site just upstream of the CT His tag coding region
• pET27 derivative; GST tag from pGEX
• T7lac promoter
• Kan resistant
• 5' cloning site: BamHI
• Low copy
• dna sequence
• MW of GST tag ~ 26kDa
• graphic map: