Sequencing Primers
The CSB stocks most (but not all) of the following. Primers for sequencing Duet coexpression vectors are listed here.
| Use |
Name |
Sequence |
Comments |
| Forward, GST fusion |
GSTseq
(pGEX-F) |
5'-GGGCTGGCAAGCCACGTTTGGTG |
Anneals near end of GST tag in pAT109/110, pBG101/105 |
| Forward, MBP fusion |
pMAL-F |
5'-GGTCGTCAGACTGTCGATGAAGCC |
Anneals near end of MBP tag in pAT107/108, pLM302/303, pSV278, pSV282 |
| Forward, Sumo fusion |
SUMOSeq |
5'-AGATTCTTGTACGACGGTATTAG |
Anneals near end of Sumo tag in pHD115, pBG102/103/104 |
| Forward, T7 |
T7 universal |
5'-TAATACGACTCACTATAGGG |
For forward sequencing of all pET-based CSB vectors. |
| Forward, Trx fusion |
Trx tag primer |
5'-GTGCACTGTCTAAAGGTCAG |
Anneals near end of Trx tag in pLM304, pNR112/113. Novagen sells this. |
| Reverse, T7 |
T7Rev |
5'-GCTAGTTATTGCTCAGCGGT |
For reverse sequencing of all CSB vectors |
|
