pSV282

N-terminal 6xHis + Maltose Binding Protein (MBP) tag; TEV protease cleavable
Optional C-terminal HSV+6xHis tags; non-cleavable
Kan resistant
pET27 derivative; MBP tag from pMAL
T7lac promoter
5' cloning site: BamHI
low copy
dna sequence
MW of His+MBP tag ~ 44kDa
graphic map: