pNR112

  • N-terminal Thioredoxin (Trx) + 6His tag; 3C protease cleavable
  • Optional C-terminal 6xHis tag; non-cleavable
  • pET21 derivative; Trx tag from pET32a
  • Amp resistant; can be used with Kan resistant host strains
  • T7lac promoter
  • 5' cloning site: BamHI
  • low copy
  • dna sequence
  • MW of Trx+His tag ~ 16 kDa
  • graphic map: