MultiColi Vectors
Designed to express multi-protein complexes in E. coli. Genes cloned into different vectors can be mixed and matched via Cre-Lox recombination.
Vector |
Size (bp) |
Type |
Resistance |
Promoter |
DNA Seq Map |
Comments |
pACE1* |
2653 |
Acceptor |
Amp |
T7 |
genbank map |
Propagate in any strain |
pACE2 |
2982 |
Acceptor |
Tet |
T7 |
genbank map |
Use DH5α or equivalent for cloning (XL1 Blues are Tet resistant). |
pACE3** |
2534 |
Acceptor |
Kan |
T7 |
genbank map |
pACE1 derivative. |
pPColi2 |
4392 |
Acceptor |
Tet |
T7 |
genbank map |
For self-cleaving polyproteins: Tev protease-your protein(s)-eCFP. |
pPColi3 |
3944 |
Acceptor |
Kan |
T7 |
genbank map |
For self-cleaving polyproteins: Tev protease-your protein(s)-eCFP. |
pDC |
2033 |
Donor |
Cap |
T7 |
genbank map |
Propagate in pir+ host strain. |
pDK |
2077 |
Donor |
Kan |
lac |
genbank map |
Propagate in pir+ host strain. |
pDS |
2027 |
Donor |
Spect |
lac |
genbank map |
Propagate in pir+ host strain. |
TevMutpDK |
2681 |
Donor |
Kan |
lac |
genbank map |
Encodes Tev protease triple mutant. Propagate in pir+ host strain. |
TevMutpDS |
2631 |
Donor |
Spect |
lac |
genbank map |
Encodes Tev protease triple mutant. Propagate in pir+ host strain. |
Amp=ampicillin; Cap=chloramphenicol; Kan= kanamycin; Spect=spectinomycin; Tet=tetracycline
*Available with NT hexaHis tag (3C or Tev protease cleavable).
**Available with NT hexaHis, decaHis, His-GST, His-MBP, His-Sumo, GST, MBP, or Trx-His tags (3C protease cleavable).
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