MultiColi Vectors

Designed to express multi-protein complexes in E. coli. Genes cloned into different vectors can be mixed and matched via Cre-Lox recombination.


Vector Size (bp) Type Resistance Promoter DNA Seq Map Comments
pACE1* 2653 Acceptor Amp T7 genbank
map		 Propagate in any strain
pACE2 2982 Acceptor Tet T7 genbank
map		 Use DH5α or equivalent for cloning (XL1 Blues are Tet resistant).
pACE3** 2534 Acceptor Kan T7 genbank
map		 pACE1 derivative.
pPColi2 4392 Acceptor Tet T7 genbank
map		 For self-cleaving polyproteins: Tev protease-your protein(s)-eCFP.
pPColi3 3944 Acceptor Kan T7 genbank
map		 For self-cleaving polyproteins: Tev protease-your protein(s)-eCFP.
pDC 2033 Donor Cap T7 genbank
map		 Propagate in pir+ host strain.
pDK 2077 Donor Kan lac genbank
map		 Propagate in pir+ host strain.
pDS 2027 Donor Spect lac genbank
map		 Propagate in pir+ host strain.
TevMutpDK 2681 Donor Kan lac genbank
map		 Encodes Tev protease triple mutant. Propagate in pir+ host strain.
TevMutpDS 2631 Donor Spect lac genbank
map		 Encodes Tev protease triple mutant. Propagate in pir+ host strain.
Amp=ampicillin; Cap=chloramphenicol; Kan= kanamycin; Spect=spectinomycin; Tet=tetracycline
*Available with NT hexaHis tag (3C or Tev protease cleavable).
**Available with NT hexaHis, decaHis, His-GST, His-MBP, His-Sumo, GST, MBP, or Trx-His tags (3C protease cleavable).