LOCUS pPColi2 4392 bp DNA circular SYN 13-SEP-2012 DEFINITION . ACCESSION pPColi2 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4392) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|GenBank 4392 bp ds-DNA circular 15-MAR-2012 ACCESSION VERSION FEATURES Location/Qualifiers misc_feature 1..1191 /label=TetRes /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Gene" /SECStyleId=1 /SECName="TetRes" misc_feature 1254..1868 /label=pBR322 origin /ApEinfo_fwdcolor=#808080 /ApEinfo_revcolor=#808080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="pBR322 origin" misc_recomb complement(2332..2365) /label=LoxP /ApEinfo_fwdcolor=#ff8040 /ApEinfo_revcolor=#ff8040 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="LoxP" promoter 2495..2511 /gene="T7" /SECDrawAs="Region" /SECStyleId=1 /SECName="T7" primer_bind 2495..2514 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Info only" /SECName="T7" misc_binding 2514..2536 /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Info only" /SECName="LacO" misc_feature 2514..2538 /gene="lac operator" /SECDrawAs="Region" /SECStyleId=1 /SECName="lac operator" misc_feature 2589..3314 /label=TEV protease /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Gene" /SECStyleId=1 /SECName="TEV protease" misc_feature 3354..3374 /label=TEV site1 /ApEinfo_fwdcolor=#feff0c /ApEinfo_revcolor=#feff0c /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="TEV site1" misc_feature 3375..4118 /label=eCFP /ApEinfo_fwdcolor=#00ff13 /ApEinfo_revcolor=#00ff13 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Gene" /SECStyleId=1 /SECName="eCFP" misc_feature 4190..4236 /label=T7Term2 /ApEinfo_fwdcolor=#800080 /ApEinfo_revcolor=#800080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="T7Term2" ORIGIN 1 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc 61 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc 121 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca 181 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta 241 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gattctctac 301 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc 361 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc 421 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttacat 481 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta 541 atgcaggagt cgcataaggg agagcgccga cccatgccct tgagagcctt caacccagtc 601 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt 661 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc 721 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc 781 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt 841 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc 901 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg 961 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc 1021 gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc 1081 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc 1141 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aaccgataca 1201 attaaaggct ccttttggag cctttttttt tggacggacc ggtagaaaag atcaaaggat 1261 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 1321 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 1381 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 1441 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 1501 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 1561 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 1621 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 1681 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 1741 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 1801 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 1861 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 1921 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 1981 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 2041 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct 2101 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt 2161 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 2221 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 2281 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gggaattcca gataacttcg 2341 tataatgtat gctatacgaa gttatggtac cgcggccgcg tagaggatct gttgatcagc 2401 agttcaacct gttgatagta cttcgttaat acagatgtag gtgttggcac catgcataac 2461 tataacggtc ctaaggtagc gacctaggta tcgataatac gactcactat aggggaattg 2521 tgagcggata acaattcccc tctagaaata attttgttta actttaagaa ggagatatac 2581 atatgggcgg agaaagcttg tttaagggac cacgtgatta caacccgata tcgagcacca 2641 tttgtcattt gacgaatgaa tctgatgggc acacaacatc gttgtatggt attggatttg 2701 gtcccttcat cattacaaac aagcacttgt ttagaagaaa taatggaaca ctgttggtcc 2761 aatcactaca tggtgtattc aaggtcaaga acaccacgac tttgcaacaa cacctcattg 2821 atgggaggga catgataatt attcgcatgc ctaaggattt cccaccattt cctcaaaagc 2881 tgaaatttag agagccacaa agggaagagc gcatatgtct tgtgacaacc aacttccaaa 2941 ctaagagcat gtctagcatg gtgtcagaca ctagttgcac attcccttca tctgatggca 3001 tattctggaa gcattggatt caaaccaagg atgggcagtg tggcagtcca ttagtatcaa 3061 ctagagatgg gttcattgtt ggtatacact cagcatcgaa tttcaccaac acaaacaatt 3121 atttcacaag cgtgccgaaa aacttcatgg aattgttgac aaatcaggag gcgcagcagt 3181 gggttagtgg ttggcgatta aatgctgact cagtattgtg ggggggccat aaagttttca 3241 tgagcaaacc tgaagagcct tttcagccag ttaaggaagc gactcaactc atgaatgaat 3301 tggtgtactc gcaaggtaac catagggata acagggtaat gcggtccggt ggtgaaaacc 3361 tgtattttca gggcgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 3421 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 3481 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 3541 cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg 3601 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 3661 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 3721 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 3781 tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca 3841 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg 3901 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 3961 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 4021 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 4081 tgtacaagtc cggactcaga tctcgacgag ctcactagct cgagagatcc ggctgctaac 4141 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc 4201 cttggggcct ctaaacgggt cttgaggggt tttttggttt aaacccatct aattggacta 4261 gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt tttctaaata 4321 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 4381 aaaaggaaga gt //