LOCUS       TevMutpDS               2631 bp    DNA     circular SYN 13-SEP-2012
DEFINITION  .
ACCESSION   TevMutpDS
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|GenBank              2631 bp ds-DNA     circular     
            21-MAR-2012
            ACCESSION   
            VERSION     
FEATURES             Location/Qualifiers
     misc_feature    51..90
                     /label=lac promoter
                     /ApEinfo_fwdcolor=#804040
                     /ApEinfo_revcolor=#804040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="lac promoter"
     misc_binding    88..110
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Info only"
                     /SECName="LacO"
     misc_feature    90..110
                     /gene="lac operator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     primer_bind     116..136
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Info only"
                     /SECName="M13-rev"
     misc_feature    187..918
                     /label=TevMut gene
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="TevMut gene"
     misc_recomb     complement(957..990)
                     /label=LoxP
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#ff8040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LoxP"
     misc_feature    992..1344
                     /label=R6Kgamma-2
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="R6Kgamma-2"
     misc_feature    1811..2602
                     /label=SpectR
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="SpectR"
ORIGIN      
        1 ctatgtcggg tgcggagaaa gaggtaatga aatggcacct aggtatcgat ggctttacac
       61 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      121 aacagctatg accatgatta cgaatttcta gaaataattt tgtttaactt taagaaggag
      181 atatacatgg gagaaagctt gtttaagggg ccgcgtgatt acaacccgat atcgagcacc
      241 atttgtcatt tgacgaatga atctgatggg cacacaacat cgttgtatgg tattggattt
      301 ggtcccttca tcattacaaa caagcacttg tttagaagaa ataatggaac actggtggtc
      361 caatcactac atggtgtatt caaggtcaag aacaccacga ctttgcaaca acacctcatt
      421 gatgggaggg acatgataat tattcgcatg cctaaggatt tcccaccatt tcctcaaaag
      481 ctgaaattta gagagccaca aagggaagag cgcatatgtc ttgtgacaac caacttccaa
      541 actaagagca tgtctagcat ggtgtcagac actagttgca cattcccttc aggcgatggc
      601 atattctgga agcattggat tcaaaccaag gatgggcagt gtggcagtcc attagtatca
      661 actagagatg ggttcattgt tggtatacac tcagcatcga atttcaccaa cacaaacaat
      721 tatttcacaa gcgtgccgaa aaacttcatg gaattgttga caaatcagga ggcgcagcag
      781 tgggttagtg gttggcgatt aaatgctgac tcagtattgt gggggggcca taaagttttc
      841 atggtgaaac ctgaagagcc ttttcagcca gttaaggaag cgactcaact catgaatgaa
      901 ttagtctact cgcaataact cgagactagt tccgtttaaa cccatgtgcc tggcagataa
      961 cttcgtataa tgtatgctat acgaagttat ggtacgtact aagctctcat gtttcacgta
     1021 ctaagctctc atgtttaacg tactaagctc tcatgtttaa cgaactaaac cctcatggct
     1081 aacgtactaa gctctcatgg ctaacgtact aagctctcat gtttcacgta ctaagctctc
     1141 atgtttgaac aataaaatta atataaatca gcaacttaaa tagcctctaa ggttttaagt
     1201 tttataagaa aaaaaagaat atataaggct tttaaagctt ttaaggttta acggttgtgg
     1261 acaacaagcc agggatgtaa cgcactgaga agcccttaga gcctctcaaa gcaattttga
     1321 gtgacacagg aacacttaac ggctgacata attcagcttc acgctgccgc aagcactcag
     1381 ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct
     1441 gaccccggat gaatgtcagc tgggaggcag aataaatgat catatcgtca attattacct
     1501 ccacggggag agcctgagca aactggcctc aggcatttga gaagcacacg gtcacactgc
     1561 ttccggtagt caataaaccg gtaagtagcg tatgcgctca cgcaactggt ccagaacctt
     1621 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     1681 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     1741 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     1801 gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     1861 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     1921 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     1981 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     2041 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     2101 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     2161 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     2221 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     2281 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     2341 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     2401 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     2461 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     2521 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     2581 caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgga t
//