LOCUS       pDS                     2027 bp    DNA     circular SYN 12-SEP-2012
DEFINITION  .
ACCESSION   pDS
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|GenBank       2027 bp ds-DNA   circular     25-OCT-2010
            ACCESSION   
            VERSION     
FEATURES             Location/Qualifiers
     misc_feature    51..90
                     /gene="lac promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="lac promoter"
     misc_binding    88..110
                     /label=misc_binding
                     /SECDrawAs="Info only"
                     /SECName="misc_binding"
     primer_bind     116..136
                     /label=primer_bind
                     /SECDrawAs="Info only"
                     /SECName="primer_bind"
     misc_feature    186..204
                     /gene="InsSeq1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq1"
     primer_bind     205..222
                     /label=primer_bind
                     /SECDrawAs="Info only"
                     /SECName="primer_bind"
     misc_feature    223..240
                     /gene="InsSeq2"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq2"
     misc_feature    260..271
                     /gene="InsSeq3"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq3"
     misc_feature    297..318
                     /gene="InsSeq4"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq4"
     misc_recomb     complement(353..386)
                     /gene="LoxP"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LoxP"
     misc_feature    388..740
                     /gene="R6kgamma ori"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="R6kgamma ori"
     misc_feature    1207..1998
                     /gene="SpectR"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="SpectR"
ORIGIN      
        1 ctatgtcggg tgcggagaaa gaggtaatga aatggcacct aggtatcgat ggctttacac
       61 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      121 aacagctatg accatgatta cgaatttcta gaaataattt tgtttaactt taagaaggag
      181 atatacatat gaggcctcgg atcctgtaaa acgacggcca gtgaattccc cgggaagctt
      241 cgccagggtt ttcccagtcg agctcgatat cggtaccagc ggataacaat ttcacatccg
      301 gatcgcgaac gcgtctcgag actagttccg tttaaaccca tgtgcctggc agataacttc
      361 gtataatgta tgctatacga agttatggta cgtactaagc tctcatgttt cacgtactaa
      421 gctctcatgt ttaacgtact aagctctcat gtttaacgaa ctaaaccctc atggctaacg
      481 tactaagctc tcatggctaa cgtactaagc tctcatgttt cacgtactaa gctctcatgt
      541 ttgaacaata aaattaatat aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta
      601 taagaaaaaa aagaatatat aaggctttta aagcttttaa ggtttaacgg ttgtggacaa
      661 caagccaggg atgtaacgca ctgagaagcc cttagagcct ctcaaagcaa ttttgagtga
      721 cacaggaaca cttaacggct gacataattc agcttcacgc tgccgcaagc actcagggcg
      781 caagggctgc taaaggaagc ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc
      841 ccggatgaat gtcagctggg aggcagaata aatgatcata tcgtcaatta ttacctccac
      901 ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca cactgcttcc
      961 ggtagtcaat aaaccggtaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     1021 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     1081 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     1141 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     1201 aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     1261 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     1321 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     1381 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     1441 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     1501 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     1561 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     1621 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     1681 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     1741 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     1801 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     1861 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     1921 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     1981 aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacggat
//