LOCUS pPColi3 3944 bp DNA circular SYN 13-SEP-2012 DEFINITION . ACCESSION pPColi3 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3944) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|GenBank 3944 bp ds-DNA circular 15-MAR-2012 ACCESSION VERSION FEATURES Location/Qualifiers CDS complement(2..817) /gene="KanR" /product="Kanamycin resistance" /label=KanR /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Gene" /SECStyleId=1 /SECName="KanR" /SECDescr="Kanamycin resistance" misc_feature 863..1545 /label=BR322 ori /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="BR322 ori" misc_recomb complement(2006..2039) /label=LoxP /ApEinfo_fwdcolor=#ff8040 /ApEinfo_revcolor=#ff8040 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="LoxP" promoter 2169..2185 /gene="T7" /product="T7 promoter" /SECDrawAs="Region" /SECStyleId=1 /SECName="T7" /SECDescr="T7 promoter" primer_bind 2169..2188 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Info only" /SECName="T7" misc_binding 2188..2210 /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Info only" /SECName="LacO" misc_feature 2188..2212 /gene="lac operator" /SECDrawAs="Region" /SECStyleId=1 /SECName="lac operator" misc_feature 2263..2988 /label=TEV protease /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Gene" /SECStyleId=1 /SECName="TEV protease" misc_feature 3028..3048 /label=TEV site1 /ApEinfo_fwdcolor=#feff0c /ApEinfo_revcolor=#feff0c /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="TEV site1" misc_feature 3049..3792 /label=eCFP /ApEinfo_fwdcolor=#00ff13 /ApEinfo_revcolor=#00ff13 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Gene" /SECStyleId=1 /SECName="eCFP" misc_feature 3864..3910 /label=T7Term2 /ApEinfo_fwdcolor=#800080 /ApEinfo_revcolor=#800080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="T7Term2" ORIGIN 1 cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa 61 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc 121 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac 181 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga 241 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc 301 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt 361 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg 421 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat 481 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat 541 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt 601 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa 661 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga 721 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg 781 gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta 841 tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt ttcgttccac 901 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 961 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 1021 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 1081 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 1141 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 1201 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 1261 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 1321 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 1381 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 1441 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 1501 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 1561 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 1621 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 1681 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat 1741 ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct gatgccgcat 1801 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca 1861 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 1921 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 1981 acgcgcgagg cagggggaat tccagataac ttcgtataat gtatgctata cgaagttatg 2041 gtaccgcggc cgcgtagagg atctgttgat cagcagttca acctgttgat agtacttcgt 2101 taatacagat gtaggtgttg gcaccatgca taactataac ggtcctaagg tagcgaccta 2161 ggtatcgata atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga 2221 aataattttg tttaacttta agaaggagat atacatatgg gcggagaaag cttgtttaag 2281 ggaccacgtg attacaaccc gatatcgagc accatttgtc atttgacgaa tgaatctgat 2341 gggcacacaa catcgttgta tggtattgga tttggtccct tcatcattac aaacaagcac 2401 ttgtttagaa gaaataatgg aacactgttg gtccaatcac tacatggtgt attcaaggtc 2461 aagaacacca cgactttgca acaacacctc attgatggga gggacatgat aattattcgc 2521 atgcctaagg atttcccacc atttcctcaa aagctgaaat ttagagagcc acaaagggaa 2581 gagcgcatat gtcttgtgac aaccaacttc caaactaaga gcatgtctag catggtgtca 2641 gacactagtt gcacattccc ttcatctgat ggcatattct ggaagcattg gattcaaacc 2701 aaggatgggc agtgtggcag tccattagta tcaactagag atgggttcat tgttggtata 2761 cactcagcat cgaatttcac caacacaaac aattatttca caagcgtgcc gaaaaacttc 2821 atggaattgt tgacaaatca ggaggcgcag cagtgggtta gtggttggcg attaaatgct 2881 gactcagtat tgtggggggg ccataaagtt ttcatgagca aacctgaaga gccttttcag 2941 ccagttaagg aagcgactca actcatgaat gaattggtgt actcgcaagg taaccatagg 3001 gataacaggg taatgcggtc cggtggtgaa aacctgtatt ttcagggcgt gagcaagggc 3061 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 3121 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 3181 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 3241 acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 3301 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 3361 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 3421 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 3481 tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac 3541 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 3601 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 3661 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 3721 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga 3781 cgagctcact agctcgagag atccggctgc taacaaagcc cgaaaggaag ctgagttggc 3841 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag 3901 gggttttttg gtttaaaccc atctaattgg actagtagcc cgcc //