LOCUS       pDC                     2033 bp    DNA     circular SYN 12-SEP-2012
DEFINITION  .
ACCESSION   pDC
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|GenBank                 2033 bp ds-DNA     circular     
            12-SEP-2012
            ACCESSION   
            VERSION     
FEATURES             Location/Qualifiers
     promoter        86..102
                     /gene="T7"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="T7"
     primer_bind     86..105
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Info only"
                     /SECName="T7"
     misc_binding    105..127
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Info only"
                     /SECName="LacO"
     misc_feature    105..129
                     /gene="lac operator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="lac operator"
     misc_feature    160..165
                     /gene="rbs"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="rbs"
     misc_feature    171..189
                     /label=InsSeq1
                     /ApEinfo_fwdcolor=#8000ff
                     /ApEinfo_revcolor=#8000ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq1"
     primer_bind     190..207
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Info only"
                     /SECName="M13-fwd"
     misc_feature    208..225
                     /label=InsSeq2
                     /ApEinfo_fwdcolor=#400080
                     /ApEinfo_revcolor=#400080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq2"
     misc_feature    245..256
                     /label=InsSeq3
                     /ApEinfo_fwdcolor=#400040
                     /ApEinfo_revcolor=#400040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq3"
     misc_feature    282..303
                     /label=InsSeq4
                     /ApEinfo_fwdcolor=#800040
                     /ApEinfo_revcolor=#800040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq4"
     misc_feature    371..417
                     /label=T7Term2
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="T7Term2"
     misc_recomb     complement(441..474)
                     /label=LoxP
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#ff8040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LoxP"
     misc_feature    527..879
                     /label=R6Kgamma-2
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="R6Kgamma-2"
     CDS             complement(1278..1934)
                     /label=CmR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="CmR"
ORIGIN      
        1 atcaacgtct cattttcgcc aaaagttggc ccagatctat gtcgggtgcg gagaaagagg
       61 taatgaaatg gcacctaggt atcgataata cgactcacta taggggaatt gtgagcggat
      121 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata catatgaggc
      181 ctcggatcct gtaaaacgac ggccagtgaa ttccccggga agcttcgcca gggttttccc
      241 agtcgagctc gatatcggta ccagcggata acaatttcac atccggatcg cgaacgcgtc
      301 tcgagagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      361 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttggtt
      421 taaacccatg tgcctggcag ataacttcgt ataatgtatg ctatacgaag ttatggtacc
      481 gcggccgcgt agaggatctg ttgatcagca gttcaacctg ttgatagtac gtactaagct
      541 ctcatgtttc acgtactaag ctctcatgtt taacgtacta agctctcatg tttaacgaac
      601 taaaccctca tggctaacgt actaagctct catggctaac gtactaagct ctcatgtttc
      661 acgtactaag ctctcatgtt tgaacaataa aattaatata aatcagcaac ttaaatagcc
      721 tctaaggttt taagttttat aagaaaaaaa agaatatata aggcttttaa agcttttaag
      781 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
      841 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acagaattag cttcacgctg
      901 ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc
      961 gcagaaacgg tgctgacccc ggatgaatgt cagctgggag gcagaataaa tgatcatatc
     1021 gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat ttgagaagca
     1081 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc
     1141 tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca
     1201 ttcatccgct tattatcact tattcaggcg tagcaaccag gcgtttaagg gcaccaataa
     1261 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1321 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1381 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1441 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     1501 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1561 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1621 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1681 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     1741 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1801 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1861 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1921 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1981 cccggtagtg atcttatttc attatggtga aagttggacc ctcttacgtg ccg
//