LOCUS       pDK                     2077 bp    DNA     circular SYN 12-SEP-2012
DEFINITION  .
ACCESSION   pDK
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|GenBank                    2077 bp ds-DNA     circular     
            22-MAR-2012
            ACCESSION   
            VERSION     
FEATURES             Location/Qualifiers
     misc_feature    51..90
                     /label=lac promoter
                     /ApEinfo_fwdcolor=#804040
                     /ApEinfo_revcolor=#804040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="lac promoter"
     misc_binding    88..110
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Info only"
                     /SECName="LacO"
     primer_bind     116..136
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Info only"
                     /SECName="M13-rev"
     misc_feature    186..204
                     /label=InsSeq1
                     /ApEinfo_fwdcolor=#8000ff
                     /ApEinfo_revcolor=#8000ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq1"
     primer_bind     205..222
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Info only"
                     /SECName="M13-fwd"
     misc_feature    223..240
                     /label=InsSeq2
                     /ApEinfo_fwdcolor=#400080
                     /ApEinfo_revcolor=#400080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq2"
     misc_feature    260..271
                     /label=InsSeq3
                     /ApEinfo_fwdcolor=#400040
                     /ApEinfo_revcolor=#400040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq3"
     misc_feature    297..318
                     /label=InsSeq4
                     /ApEinfo_fwdcolor=#800040
                     /ApEinfo_revcolor=#800040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="InsSeq4"
     misc_recomb     complement(353..386)
                     /label=LoxP
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#ff8040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LoxP"
     misc_feature    388..740
                     /label=R6Kgamma3
                     /ApEinfo_fwdcolor=#0000a0
                     /ApEinfo_revcolor=#0000a0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="R6Kgamma3"
     misc_feature    1105..1899
                     /label=KanR2
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="KanR"
ORIGIN      
        1 ctatgtcggg tgcggagaaa gaggtaatga aatggcacct aggtatcgat ggctttacac
       61 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      121 aacagctatg accatgatta cgaatttcta gaaataattt tgtttaactt taagaaggag
      181 atatacatat gaggcctcgg atcctgtaaa acgacggcca gtgaattccc cgggaagctt
      241 cgccagggtt ttcccagtcg agctcgatat cggtaccagc ggataacaat ttcacatccg
      301 gatcgcgaac gcgtctcgag actagttccg tttaaaccca tgtgcctggc agataacttc
      361 gtataatgta tgctatacga agttatggta cgtactaagc tctcatgttt cacgtactaa
      421 gctctcatgt ttaacgtact aagctctcat gtttaacgaa ctaaaccctc atggctaacg
      481 tactaagctc tcatggctaa cgtactaagc tctcatgttt cacgtactaa gctctcatgt
      541 ttgaacaata aaattaatat aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta
      601 taagaaaaaa aagaatatat aaggctttta aagcttttaa ggtttaacgg ttgtggacaa
      661 caagccaggg atgtaacgca ctgagaagcc cttagagcct ctcaaagcaa ttttcagtga
      721 cacaggaaca cttaacggct gacagaatta gcttcacgct gccgcaagca ctcagggcgc
      781 aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
      841 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
      901 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
      961 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     1021 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa
     1081 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     1141 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     1201 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     1261 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     1321 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     1381 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     1441 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     1501 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     1561 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     1621 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacacatgg cgatgcctgc
     1681 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     1741 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     1801 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     1861 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     1921 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     1981 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     2041 gggatctcat gctggagttc ttcgcccacc ccgggat
//