DNA Transactions at an Atomic Level

The genetic information encoded within DNA is copied, maintained, and decoded by protein machines. Our laboratory uses X-ray crystallography and other high-resolution structural and biochemical approaches to investigate the molecular details of how these proteins repair damaged DNA and carry out DNA synthesis.

Featured Article

Structure of a DNA glycosylase that unhooks interstrand cross-links. Proc.Natl.Acad.Sci.,USA, 114: 4400-4405

In the News

VICB feature of our recent PNAS paper on AlkZ

Prof. Eichman featured in SBGrid Member Tales

Zack receives NIH postdoctoral fellowship

Vanderbilt story on Elwood's Nature paper

Diana's work on HLTF described in VUMC Reporter

News Archives

Training Opportunities Available

  • Time-resolved crystallography to monitor base excision repair by DNA glycosylases
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  • HLTF's ancient HIRAN domain binds 3' DNA ends to drive replication fork reversal
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  • Bacterial AlkD is the first glycosylase discovered to repair a bulky lesions like the natural product yatakemycin
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  • How do DNA repair enzymes search the genome for chemical damage?

  • How are stalled replication forks repaired?

  • SMARCAL1 HARP domain is important for reversal of stalled forks
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  • HEAT repeats have emerged as an important nucleic acid binding architecture

  • Base flipping is not a prerequisite for excision repair by DNA glycosylases
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  • CH-π interactions important for catalysis of base excision by DNA glycosylase AlkD
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  • Crystals used to determine the atomic structures of a protein-DNA complex

  • X-ray diffraction image from a protein crystal

  • Building the atomic structure of a protein-DNA complex into experimental electron density from X-ray diffraction data

  • NMR chemical shift perturbation to monitor protein structural changes induced by DNA binding
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  • Structural studies of the vertebrate replisome
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  • E. coli AidB is a DNA binding protein involved in the bacterial adaptive response to alkylating agents
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