Our Stocks

Our Proteins

• Annexin XI stocks
• Calbindin stocks
• Calcyclin stocks
• Calmodulin stocks
• Caltractin stocks
• MRP8/14 stocks
• S100A2 Stocks
• Stellacyanin stocks
• Troponin C stocks

Other Stocks

• Cell stocks
• Plasmid stocks
• DNA from Kuznicki lab

Laboratory Procedures

Protocol template file

General Procedures

• Standard Cell Prep
• Preparation of Competent Cells (old)
• Getting DNA sequenced
• Isolating Inclusion Bodies
• MALDI
• Amino Acid Analysis
• Minimal Media Recipe
• Sterile Technique
• Thrombin Cleavage
• Western Blotting and Immunostaining
• Limited proteolysis
• Thermal melt using CD
• Calculating denaturant concentration by r.i.
• Theoretical simulations and considerations for Kd measurement and calculation
• Protocol for growing labeled protein

DNA Processing Assemblies

• RPA-AB Purification
• T-Antigen Purification
• DNA primase Exp. / Purification

Calcium-Binding Proteins

• Calbindin D_9k Prep
• updated Calbindin D_9k Prep
• Calmodulin Prep
• S100A2 Prep
• S100A6 Prep
• S100B Prep
• S100A8/A9 Prep
• Production of Calbindomodulin Mutants
• Centrin Prep
• Purification Protocol for HCentrin2
• CDPK prep
• Competitive Chelator Method for Calcium-Binding Constants (Updated version)
• Phenyl Sepharose Binding Assay for Calbindin Mutants
• Preparing Chelexed water
• Walter's notes about NMR with EDTA

NMR and Computational Wisdom

• Beginner's guide to data collection on Bruker instruments using xwinnmr

FELIX

• Introduction to Felix
• Useful but undocumented Felix97 commands
• BioMagResBank info
• Grad2- Shuffle gradient echo/antiecho data into sine-cosine (States) format
• sshift - Calculate secondary chemical shifts 
• Automation of GENXPK tasks from within Felix
• Assigning crosspeaks with GENXPK
• Customizing the Felix "right-click" menu
• Linear prediction do's and don'ts
• Linear prediction parameters
• Programming Felix95 hotkeys
• Enabling keypad navigation in Felix95 on SUN computers

Other NMR Data Processing

• Removing off-resonance water line
• Handling ¹³C resonances at spectrum edge
• Tips on getting Modelfree up and running
• Overlaying multiple spectra in colors
• Linse Lab's titration analysis program
• VUBNMR Spectrometer Updates
• Summary of common commands used in Bruker pulse programming
• Description of some automated backbone and NOE assignment packages

Calculating Structures

"Overall concept good, details very dated"
• Chazin Lab "how to"

Analysis and Comparison of Structures

• Contact maps with CHARMM
• Recommendations for the Presentation of NMR Structures of Proteins and Nucleic Acids-PDF format

Software Manuals

• suppose - RMSD calculations and multiple superpositions of NMR structures
• ACE/gr-also known as xmgr or xvgr
• MolScript
• Mathematica

Javascript and Perl Utilities

"Concepts good, but details dated"
• Perl scripts for use in structure calculations