Evolutionary Relationships of Other Calcium-Binding Proteins

The information presented here was taken from Nakayama,S., N. Moncrief, and R. Kretsinger, (1992) J. Mol. Evol. 34, 416-448 and Nakayama, S. and R. Kretsinger (1994) Annu. Rev. Biophys. Biomol. struct. 23, 473,507. However, any inaccuracies are my own.

parvalbumin has three EF hands (EF hands 1,2, and 3). However, the first EF hand does not bind calcium. The first two EF hands branch from EF3, and the third one branches from EF4.
calbindin D(28k) and calretinin are congruent. They seem to have evolved from a two hand precursor. The current proteins with six hands were reached via duplication and fusion. Therefore, hands 1,3, and 5 are closely related, as are hands 2,4, and 6. The precursor to hands 1, 3, and 5 branched from EF3. The precursor to hands 2, 4, and 6 branched from EVEN.