Rosetta Antibody Workshop - April/May 2019
Preparing for the Workshop
UNIX Operating Systems
Workshop participants are expected to be familiar with UNIX-like operating systems. Before the workshop, please review the following resources:
For those who wish to improve their UNIX usage skills, there is an optional "Introduction to Linux" session the first evening.
Scripting Languages
Rosetta users often prepare input files and analyze Rosetta output models using scripts written in Python or Perl.
We recommend familiarizing yourself with Python:
Molecular Visualization Tools
You will also need a tool to visualize biomolecular structures. We will assume the use of PyMOL, but you can use any of the tools below:
Rosetta Resources
For the tutorials, we will provide computers which have Rosetta already installed and ready to run.
However, if you wish to also install Rosetta on your own computer, you can obtain a license and download Rosetta from the RosettaCommons website.
(Note that Rosetta does not run natively on Windows systems.)
You can learn more about Rosetta using these resources:
The tutorials are based in part on the following papers:
- Kaufmann, K. W., Lemmon, G. H., Deluca, S. L., Sheehan, J. H., & Meiler, J. (2010). Practically useful: what the Rosetta protein modeling suite can do for you. Biochemistry, 49(14), 2987-2998.
- Bender BJ, Cisneros A, Duran AM, Finn JA, Fu D, Lokits AD, Mueller BK, Sangha AK, Sauer MF, Sevy AM, Sliwoski G, Sheehan JH, DiMaio F, Meiler J, & Moretti R. (2016). Protocols for Molecular Modeling with Rosetta3 and RosettaScripts. Biochemistry, 55(34), 4748-63.
Materials from previous workshops can be dowloaded from the Meiler Lab website.
Additional demos can be found here.
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