Chazin Home | Ca-binding Protein DB | Vanderbilt Home
	Research Description | Publications | Wisdom | Search

J. Am. Chem. Soc. 120, 607-608 (1998) Full Article | Supplementary Material

Enzymatic synthesis of region-specific isotope-labeled DNA oligomers for NMR analysis.

Mer G, Chazin WJ

Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

A general method is described for large scale preparation of 13C and 15N region-specific enriched DNA oligomers that can be used for NMR studies. The procedure combines two enzymatic polymerisation reactions: first of 13C,15N-labeled and then of nonlabeled deoxyribonucleotides. It is applied to the regional labeling of one 16mer strand of a 64 residue four-arm DNA structure that serves as a model for the Holliday junction intermediate in genetic recombination and repair.

Full Article | Supplementary Material