LOCUS pETDuet_1 5420 bp ds-DNA circular 20-MAY-2013 DEFINITION pETDUET-1 SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:0 FEATURES Location/Qualifiers RBS 1548..1553 /vntifkey="32" /label=RBS-1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 -35_signal complement(3532..3537) /vntifkey="48" /label=-35\Ap /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 -10_signal complement(3508..3514) /vntifkey="47" /label=-10\Ap /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 2037..2484 /vntifkey="33" /label=f1\ori /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 4227..4227 /vntifkey="33" /label=ColE1,\pBR322\ori /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(4658..4849) /vntifkey="4" /label=ROP /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind 1495..1515 /vntifkey="31" /label=lacO /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 terminator 1952..1999 /vntifkey="43" /label=T7\terminator /ApEinfo_fwdcolor=#ff1e9e /ApEinfo_revcolor=#ff1e9e /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(2606..3466) /vntifkey="4" /note="B-lactamase gene" /label=Ap /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 1573..1590 /vntifkey="20" /label=His-Tag /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind 1856..1900 /vntifkey="31" /label=S-Tag /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind 1725..1745 /vntifkey="31" /label=lacO(1) /ApEinfo_label=lacO /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 1704..1720 /vntifkey="30" /label=T7\promoter /ApEinfo_fwdcolor=#007cff /ApEinfo_revcolor=#007c00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 RBS 1776..1781 /vntifkey="32" /label=RBS /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer 1679..1698 /vntifkey="27" /label=DuetUP2\Primer\#71180-3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer complement(1679..1698) /vntifkey="27" /label=DuetDOWN1\Primer\#71179-3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer complement(1938..1956) /vntifkey="27" /label=T7\Terminator\Primer\#69337-3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(1..1083) /vntifkey="4" /note="lac repressor gene" /label=lacI /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 1474..1490 /vntifkey="30" /label=T7\promoter(1) /ApEinfo_label=T7\promoter /ApEinfo_fwdcolor=#007cff /ApEinfo_revcolor=#007c00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer 1428..1443 /vntifkey="27" /label=pET\Upstream\Primer\#69214-3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga 121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc 181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata 241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac caacgcgcag 301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat 361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc 421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg 481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat 541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga 601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt 661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag 721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct 781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc 841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa 901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat 961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg 1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt 1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt 1141 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta 1201 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat 1261 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga 1321 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga 1381 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt 1441 agaggatcga gatcgatctc gatcccgcga aattaatacg actcactata ggggaattgt 1501 gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc 1561 atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag ctcggcgcgc 1621 ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag taatcgtatt 1681 gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt gagcggataa 1741 caattcccca tcttagtata ttagttaagt ataagaagga gatatacata tggcagatct 1801 caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt ctggtaaaga 1861 aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg cagcttaatt 1921 aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg 1981 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg 2041 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 2101 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 2161 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 2221 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 2281 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 2341 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 2401 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 2461 aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg agattatcaa 2521 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 2581 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 2641 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 2701 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 2761 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 2821 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 2881 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 2941 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 3001 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 3061 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 3121 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 3181 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 3241 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 3301 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 3361 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 3421 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 3481 aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 3541 atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt tttcgttcca 3601 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 3661 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 3721 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 3781 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 3841 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 3901 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 3961 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 4021 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 4081 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 4141 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 4201 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 4261 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 4321 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 4381 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 4441 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc 4501 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 4561 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 4621 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 4681 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga 4741 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc 4801 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg 4861 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca 4921 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac 4981 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg 5041 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga 5101 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga 5161 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc 5221 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg 5281 tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg aaggagctga 5341 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac 5401 ttacattaat tgcgttgcgc //