Before submitting samples, please follow the instructions on page 2.
The sequencing success depends on you adhering to these instructions.

Vanderbilt University DNA Sequencing Facility                   FORM #4
531 LH									     Effective July 28 2003
Phone: 936-2086
Sequencing Reaction and Run Request

User Information: (Fill in all the boxes)

User First Name:	Jonathan	PI First Name:	Walter	
User Last Name:	Sheehan	PI Last Name:	Chazin	
Email:	Jonathan.Sheehan@vanderbilt.edu	Department:	Biochem	
Phone:	6-2233	Date:	5-19-04	

DNA Type: (Type an X in the box)
Plasmid    
(25ng/ul)	PCR Product 
 (25-50ng/ul)	Cosmid or BAC
(65ng/ul)	Full Plate
(96 reactions)	
X				

Vector Name 
(for cloned DNA):
pSV271 (pET27 based)	

Sample information:

a.		Write only ONE DNA/Primer combination in each line.
b.		Sample names on this sheet should exactly match with the sample tubes.

	Customer Code	DNA Name	DNA Size	DNA 	Primer Name	Primer	Center Number	
	user initials  PI initials
Ex. DTBALG	< 8 characters total	< 3kb, 3-10kb,
or  >10kb	CONC.
REQUIRED	< 6 characters total	Conc.
(10ng/ul)	REQUIRED
Ex. 4-04-123-4567	
 1	JHSWJC	1CBM2PM	<3kb	25	T7		4-04-253-0071	
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Note: We stock the following sequencing primers: M13Forward, M13Reverse, SP6, T3, and T7.
Please do not bring these primers.