For expression of N-terminally His-tagged human rhinovirus 3C protease in E. coli.
MGSSHHHHHH GGGLVPRGPN TEFALSLLRK NIMTITTSKG EFTGLGIHDR VCVIPTHAQP
GDDVLVNGQK IRVKDKYKLV DPENINLELT VLTLDRNEKF RDIRGFISED LEGVDATLVV
HSNNFTNTIL EVGPVTMAGL INLSSTPTNR MIRYDYATKT GQCGGVLCAT GKIFGIHVGG
- pET derivative
- T7lac promoter
- low copy
- Kan resistance
- 3C protease recognition sequence: LEVLFQ↓GP
- MW = 21.8 kDa
- Amino acid sequence:
Protocol: 3C protease expression and purification
This document also contains some information on buffer components that affect protease activity.