InfoCard 50	About InfoCards

Reference:	Linse, S., Johansson, C., Brodin, P., Grundstrom, T., Drakenberg, T., Forsen, S. Electrostatic contributions to the binding of Ca(2+) in calbindin D9k (1991) Biochemistry 30, 154-183.
Deposited by:	Melanie R. Nelson, The Scripps Research Institute, Molecular Biology
Depositor's e-mail address:	mnelson@scripps.edu
Date deposited:	06-18-1998
Validated by:	Melanie R. Nelson (mnelson@scripps.edu)
Comments:	The calcium affinity was determined using the competitive chelator method. The chelator used was Quin2. Protein concentrations were between 20 and 30 micromolar (the exact concentration is determined as part of the fitting of the titration curve.)