Reference:
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Linse, S., Johansson, C., Brodin, P., Grundstrom, T., Drakenberg, T., Forsen, S. Electrostatic contributions to the binding of Ca(2+) in calbindin D9k (1991) Biochemistry 30, 154-183.
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Deposited by:
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Melanie R. Nelson, The Scripps Research Institute, Molecular Biology
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Depositor's e-mail address:
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mnelson@scripps.edu
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Date deposited:
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02-17-1998
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Validated by:
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Melanie R. Nelson (mnelson@scripps.edu)
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Comments:
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The calcium affinity was determined using the competitive chelator method. The chelator used was Quin2. An earlier report from the same lab gives slightly different calcium binding constants. This is because the earlier report used partially deamidated protein. The reference for the earlier report is: Linse, S., T. Drakenberg, E. Thulin, P. Sellers, K. Elmden, T. Grundstrom, S. Forsen. Structure-function relationships in EF-Hand Ca2+-binding proteins. Protein engineering and biophysical studies of calbindin D9k (1987) Biochemistry. 26, 6723-6735.
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