Information in the EF-Hand Calcium-Binding Proteins Database from Reference 638

Information in the EF-Hand Calcium-Binding Proteins Database from Reference 638

Protein	Species	Information	Submitted By	Validated By
calbindin D9k mutant: P43M L6V	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F10A	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23A	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23G	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L28A	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61A	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66W	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66A	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V70L	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M I73V	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61G	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M this mutant is often used in NMR studies to substitute for wildtype, because it removes the problem of two sets of signals arising due to cis-trans isomerization at P43	Bos taurus (cow or bovine)	This protein binds 2 equivalents of Ca ion	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L6V	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 1.66E08 K₂ = 3.63E08 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F10A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 6.92E06 K₂ = 1.86E07 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 9.12E06 K₂ = 9.55E07 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23G	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 3.89E06 K₂ = 3.24E06 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L28A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 2.75E07 K₂ = 9.33E06 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 5.89E07 K₂ = 1.12E07 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66W	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 5.25E08 K₂ = 1.05E09 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 1.15E07 K₂ = 7.59E07 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V70L	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 1.17E08 K₂ = 1E08 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M I73V	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 9.12E07 K₂ = 5.25E08 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61G	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 5.89E06 K₂ = 2.95E08 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M this mutant is often used in NMR studies to substitute for wildtype, because it removes the problem of two sets of signals arising due to cis-trans isomerization at P43	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 5.62E07 K₂ = 3.89E08 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L6V	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 6.03E07 K₂ = 1.05E08 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F10A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 2.51E06 K₂ = 1.26E07 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 3.16E06 K₂ = 4.27E07 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23G	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 912000 K₂ = 2E06 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L28A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 4.47E06 K₂ = 4.9E06 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 1.32E07 K₂ = 4.57E06 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66W	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 1.86E08 K₂ = 2.45E08 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 4.79E06 K₂ = 4.27E07 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V70L	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 3.09E07 K₂ = 3.16E07 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M I73V	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 3.16E07 K₂ = 1.05E07 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61G	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 2.24E06 K₂ = 1E08 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M this mutant is often used in NMR studies to substitute for wildtype, because it removes the problem of two sets of signals arising due to cis-trans isomerization at P43	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 1.48E07 K₂ = 1.1E08 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L6V	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 9.55E06 K₂ = 1.29E07 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F10A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 692000 K₂ = 2.88E06 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 490000 K₂ = 1.48E07 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23G	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 158000 K₂ = 1.23E06 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L28A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 724000 K₂ = 1.38E06 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 2.04E06 K₂ = 977000 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66W	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 2.88E07 K₂ = 2.24E07 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 631000 K₂ = 1.58E07 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V70L	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 4.37E06 K₂ = 4.79E06 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M I73V	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 7.24E06 K₂ = 1.58E07 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61G	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 316000 K₂ = 2E07 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M this mutant is often used in NMR studies to substitute for wildtype, because it removes the problem of two sets of signals arising due to cis-trans isomerization at P43	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 2.75E06 K₂ = 1.29E07 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L6V	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 3.02E06 K₂ = 3.16E06 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F10A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 275000 K₂ = 813000 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 126000 K₂ = 6.31E06 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23G	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 53700 K₂ = 631000 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L28A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 229000 K₂ = 501000 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 676000 K₂ = 389000 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66W	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 8.91E06 K₂ = 7.41E06 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66A	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 347000 K₂ = 4.07E06 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V70L	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 1.26E06 K₂ = 1.38E06 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M I73V	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 2.04E06 K₂ = 3.16E06 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61G	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 100000 K₂ = 1E07 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M this mutant is often used in NMR studies to substitute for wildtype, because it removes the problem of two sets of signals arising due to cis-trans isomerization at P43	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 955000 K₂ = 3.16E06 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu