| Protein |
Species |
Information |
Comment |
Submitted By |
Validated By |
|---|---|---|---|---|---|
| calmodulin isoform 1 the main isoform, and the only isoform found in most species |
Homo sapiens (human) |
Report of an engineered mutant with following mutations:
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calmodulin isoform 1 mutant:
|
Homo sapiens (human) |
This mutation causes a decrease in the thermal stability of the isolated Cam-C domain, as indicated by a 11.8 degree Celsius shift in the far-UV CD thermal dentauration curve. It also increases the calcium affinity. The calcium-dependent increase in bisANS fluorescence is 45% less in CaM-Ca with the F92A mutation than in wildtype CaM-C. This fluorescence increase monitors how hydrophobic the environment of the probe is-- a larger increase corresponds to a more hydrophobic environment | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |