| Protein |
Species |
Information |
Comment |
Submitted By |
Validated By |
|---|---|---|---|---|---|
| calbindin D9k |
Bos taurus (cow or bovine) |
This protein binds 2 equivalents of Ca ion | This paper reports the calcium affinity of wildtype calbindin D9k and of several mutants |
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
| calbindin D9k |
Bos taurus (cow or bovine) |
Report of an engineered mutant with following mutations:
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
This protein binds 2 equivalents of Ca ion | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Ca binding constants measured by competitive chelator:
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
This protein binds 2 equivalents of Ca ion | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k |
Sus scrofa (pig) |
This protein binds 2 equivalents of Ca ion | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k |
Sus scrofa (pig) |
Ca binding constants measured by competitive chelator:
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
This protein binds 2 equivalents of Ca ion | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Ca binding constants measured by competitive chelator:
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
This protein binds 2 equivalents of Ca ion | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Ca binding constants measured by competitive chelator:
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Apo form is slightly less stable to urea and temperature unfolding compared to wildtype | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Macroscopic calcium binding constants are lower than for wildtype calbindin, particularly K2 | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Macroscopic calcium binding constants are lower than for wildtype calbindin, particularly K2 | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Macroscopic binding constants are lower than for wildtype calbindin, particularly K2. Interestingly, K1 is slightly higher than K1 for P20G alone, while K2 is about the same (within error bars) | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |