CaBP Data Library General Information

Information in the EF-Hand Calcium-Binding Proteins Database from Reference 211

Protein
Species
Information
Comment
Submitted By
Validated By
calbindin D9k
mutant:
  • A14 deleted
  • A15D
  • P20G
  • P43M
  • N21 deleted
Bos taurus
(cow or bovine)
This protein binds 2 equivalents of Ca ion
Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
Melanie R. Nelson
mnelson@scripps.edu
calbindin D9k
mutant:
  • A14 deleted
  • A15D
  • P20G
  • P43M
  • N21 deleted
Bos taurus
(cow or bovine)
Ca binding constants measured by competitive chelator:
  • K1 = 1.2E08 +/- 2E07
  • K2 = 1.2E07 +/- 2E06
  • Temperature: 25
  • pH: 7.5
  • Salt concentration: no salt added
  • Buffer:
    • 0.002 M Tris-HCl
The chelator used was Quin2
Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
Melanie R. Nelson
mnelson@scripps.edu