Calbindin D9k Mutant: P20 deleted
Engineered mutant of calbindin D9k, from Bos taurus (InfoCard)
Similar Mutants:
General Effects of the Mutation:
- Apo state is slightly less stable to urea denaturation than apo state of wildtype is. Apo is state is also slightly less stable than apo state of P20G (InfoCard)
- Macroscopic calcium binding constants are lower than for wildtype calbindin, particularly K2 (InfoCard)
Physical Properties of the Mutant:
Stability:
Free energy of unfolding = 3.58 +/- 0.24 kcal/mol
cooperativity of unfolding = 0.86 kcal/(mol M)
(measured by: urea denaturation pH: 7.1 temperature: 25 degrees Celsius )
(InfoCard)
Ca Binding Constants (low salt:) :
K1 = 8 x 107 +/- 1.5 x 107; K2 = 5.00 x 105 +/- 2.00 x 105
(measured by: competitive chelator pH: 7.5 temperature: 25 degrees Celsius buffer: 0.002 M Tris-HCl )
(InfoCard)