CaBP

Calbindin D9k Mutant: P20G

Engineered mutant of calbindin D9k, from Bos taurus (InfoCard)


Similar Mutants:


General Effects of the Mutation:


Physical Properties of the Mutant:

Stability:
Free energy of unfolding = 3.58 +/- 0.24 kcal/mol
cooperativity of unfolding = 0.788 kcal/(mol M)
(measured by: urea denaturation pH: 7.1 temperature: 25 degrees Celsius buffer: 0.001 M EGTA, 0.02 M Pipes )
(InfoCard)

Ca Binding Constants (low salt:) :

K1 = 6.57 x 107 +/- 1.5 x 107; K2 = 8 x 106 +/- 6 x 106
(measured by: competitive chelator pH: 7.5 temperature: 25 degrees Celsius buffer: 0.002 M Tris-HCl )
(InfoCard)