Calbindin D9k Mutant: E17Q
Engineered mutant of calbindin D9k, from Bos taurus (InfoCard)
General Effects of the Mutation:
- Apo state is slightly more stable to urea denaturation than wildtype apo state is, probably due to a decrease in electrostatic repulsion at the binding site (InfoCard)
Physical Properties of the Mutant:
Stability:
Free energy of unfolding = 5.26 +/- 0.24 kcal/mol
cooperativity of unfolding = 0.908 kcal/(mol M)
(measured by: urea denaturation pH: 7.1 temperature: 25 degrees Celsius buffer: 0.001 M EGTA, 0.02 M Pipes )
(InfoCard)
Ca Binding Constants (low salt:) :
K1 = 2.5 x 107; K2 = 1.26 x 108
(measured by: competitive chelator pH: 7.5 temperature: 25 degrees Celsius buffer: 0.002 M Tris-HCl )
(InfoCard)
Ca Binding Constants (medium salt:) :
K1 = 3.16 x 106; K2 = 1 x 107
(measured by: competitive chelator pH: 7.5 temperature: 25 degrees Celsius salt: 50 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)
Ca Binding Constants (medium salt:) :
K1 = 2 x 106; K2 = 3.16 x 106
(measured by: competitive chelator pH: 7.5 temperature: 25 degrees Celsius salt: 100 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)
Ca Binding Constants (high salt:) :
K1 = 1.58 x 106; K2 = 2.51 x 106
(measured by: competitive chelator pH: 7.5 temperature: 25 degrees Celsius salt: 150 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)