CaBP

Calbindin D9k Mutant: P43M

Engineered mutant of calbindin D9k, from Bos taurus (InfoCard)

this mutant is often used in NMR studies to substitute for wildtype, because it removes the problem of two sets of signals arising due to cis-trans isomerization at P43

Similar Mutants:

Physical Properties of the Mutant:

Stability:
Free energy of unfolding = 6.55 +/- 0.31 kcal/mol
cooperativity of unfolding = 1.17 kcal/(mol M)
(measured by: urea denaturation pH: 7 temperature: 25 degrees Celsius buffer: 0.0005 M EGTA, 0.01 M potassium phosphate )
(InfoCard)

Ca Binding Constants (low salt:) :

K1 = 5.62 x 107; K2 = 3.89 x 108
(measured by: competitive chelator pH: 7.5 buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 1.48 x 107; K2 = 1.1 x 108
(measured by: competitive chelator pH: 7.5 salt: 10 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 2.75 x 106; K2 = 1.29 x 107
(measured by: competitive chelator pH: 7.5 salt: 50 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (high salt:) :

K1 = 9.55 x 105; K2 = 3.16 x 106
(measured by: competitive chelator pH: 7.5 salt: 150 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)