CaBP

Calbindin D9k Mutant: P43M + I73V

Engineered mutant of calbindin D9k, from Bos taurus (InfoCard)

Similar Mutants:

Physical Properties of the Mutant:

Stability:
Free energy of unfolding = 5.02 +/- 0.26 kcal/mol
cooperativity of unfolding = 1.15 kcal/(mol M)
(measured by: urea denaturation pH: 7 temperature: 25 degrees Celsius buffer: 0.0005 M EGTA, 0.01 M potassium phosphate )
(InfoCard)

Ca Binding Constants (low salt:) :

K1 = 9.12 x 107; K2 = 5.25 x 108
(measured by: competitive chelator pH: 7.5 buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 3.16 x 107; K2 = 1.05 x 107
(measured by: competitive chelator pH: 7.5 salt: 10 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 7.24 x 106; K2 = 1.58 x 107
(measured by: competitive chelator pH: 7.5 salt: 50 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (high salt:) :

K1 = 2.04 x 106; K2 = 3.16 x 106
(measured by: competitive chelator pH: 7.5 salt: 150 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)