CaBP

Calbindin D9k Mutant: P43M + F66A

Engineered mutant of calbindin D9k, from Bos taurus (InfoCard)

Similar Mutants:

Physical Properties of the Mutant:

Stability:
Free energy of unfolding = 1.58 +/- 0.12 kcal/mol
cooperativity of unfolding = 0.79 kcal/(mol M)
(measured by: urea denaturation pH: 7 temperature: 25 degrees Celsius buffer: 0.0005 M EGTA, 0.01 M potassium phosphate )
(InfoCard)

Ca Binding Constants (low salt:) :

K1 = 1.15 x 107; K2 = 7.59 x 107
(measured by: competitive chelator pH: 7.5 buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 4.79 x 106; K2 = 4.27 x 107
(measured by: competitive chelator pH: 7.5 salt: 10 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 6.31 x 105; K2 = 1.58 x 107
(measured by: competitive chelator pH: 7.5 salt: 50 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (high salt:) :

K1 = 3.47 x 105; K2 = 4.07 x 106
(measured by: competitive chelator pH: 7.5 salt: 150 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)