CaBP

Calbindin D9k Mutant: P43M + F66W

Engineered mutant of calbindin D9k, from Bos taurus (InfoCard)

Similar Mutants:

Physical Properties of the Mutant:

Stability:
Free energy of unfolding = 4.2 +/- 0.33 kcal/mol
cooperativity of unfolding = 0.69 kcal/(mol M)
(measured by: urea denaturation pH: 7 temperature: 25 degrees Celsius buffer: 0.0005 M EGTA, 0.01 M potassium phosphate )
(InfoCard)

Ca Binding Constants (low salt:) :

K1 = 5.25 x 108; K2 = 1.05 x 109
(measured by: competitive chelator pH: 7.5 buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 1.86 x 108; K2 = 2.45 x 108
(measured by: competitive chelator pH: 7.5 salt: 10 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 2.88 x 107; K2 = 2.24 x 107
(measured by: competitive chelator pH: 7.5 salt: 50 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (high salt:) :

K1 = 8.91 x 106; K2 = 7.41 x 106
(measured by: competitive chelator pH: 7.5 salt: 150 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)