CaBP

Calbindin D9k Mutant: L28A + P43M

Engineered mutant of calbindin D9k, from Bos taurus (InfoCard)

Similar Mutants:

Physical Properties of the Mutant:

Stability:
Free energy of unfolding = 3.85 +/- 0.17 kcal/mol
cooperativity of unfolding = 1.03 kcal/(mol M)
(measured by: urea denaturation pH: 7 temperature: 25 degrees Celsius buffer: 0.0005 M EGTA, 0.01 M potassium phosphate )
(InfoCard)

Ca Binding Constants (low salt:) :

K1 = 2.75 x 107; K2 = 9.33 x 106
(measured by: competitive chelator pH: 7.5 buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 4.47 x 106; K2 = 4.9 x 106
(measured by: competitive chelator pH: 7.5 salt: 10 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 7.24 x 105; K2 = 1.38 x 106
(measured by: competitive chelator pH: 7.5 salt: 50 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (high salt:) :

K1 = 2.29 x 105; K2 = 5.01 x 105
(measured by: competitive chelator pH: 7.5 salt: 150 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)