CaBP

Calbindin D9k Mutant: L23G + P43M

Engineered mutant of calbindin D9k, from Bos taurus (InfoCard)

Similar Mutants:

Physical Properties of the Mutant:

Stability:
Free energy of unfolding = 2.27 +/- 0.31 kcal/mol
cooperativity of unfolding = 1.22 kcal/(mol M)
(measured by: urea denaturation pH: 7 temperature: 25 degrees Celsius buffer: 0.0005 M EGTA, 0.01 M potassium phosphate )
(InfoCard)

Ca Binding Constants (low salt:) :

K1 = 3.89 x 106; K2 = 3.24 x 106
(measured by: competitive chelator pH: 7.5 buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 9.12 x 105; K2 = 2 x 106
(measured by: competitive chelator pH: 7.5 salt: 10 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (medium salt:) :

K1 = 1.58 x 105; K2 = 1.23 x 106
(measured by: competitive chelator pH: 7.5 salt: 50 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)

Ca Binding Constants (high salt:) :

K1 = 5.37 x 104; K2 = 6.31 x 105
(measured by: competitive chelator pH: 7.5 salt: 150 mM KCl buffer: 0.002 M Tris-HCl )
(InfoCard)