Calmodulin Mutant: I85C + L112C

Engineered mutant of isoform 1 calmodulin, from Homo sapiens (InfoCard)

General Effects of the Mutation:

• This mutant forms a disulfide bond that prevents helices III and IV of the C domain from moving apart. This mutant shows decreases Ca2+ affinity in the C domain binding loops, as followed by intrinsic tyrosine fluorescence. It partially recovers activity when the disulfide bond is reduced and the cysteines blocked by cyanylation (InfoCard)

Physical Properties of the Mutant:

No physical properties of this mutant are currently stored in the database

Target Activation