Information from the EF-Hand CaBP Database about Calmodulin Mutant I125M

Engineered mutant of isoform 1 calmodulin, from Bos taurus (InfoCard)

Shows wildtype ability to activate myosin light chain kinase (103% of max), but reduced ability to activate nitric oxide synthase (84% of max) (InfoCard)

No physical properties of this mutant are currently stored in the database

	Target Protein	Acitivation Ability	Percent Activation (compared to wt)	K(act) (nM)
I125M	smooth muscle myosin light chain kinase	yes (InfoCard)	103 % (InfoCard)
wildtype from Bos taurus		yes (InfoCard)	100 % (InfoCard)	1.5 (InfoCard)

I125M	neuronal nitric oxide synthase	partial (InfoCard)	84 % (InfoCard)