Information from the EF-Hand CaBP Database about Calmodulin Mutant M124L

Engineered mutant of isoform 1 calmodulin, from Bos taurus (InfoCard)

Shows reduced ability to activate myosin light chain kinase (81% of max) and nitric oxide synthase (65% of max) (InfoCard)
Activation of PDE by this mutant is nearly indistinguishable from that acheived by wildtype CaM (InfoCard)

No physical properties of this mutant are currently stored in the database

	Target Protein	Acitivation Ability	Percent Activation (compared to wt)	K(act) (nM)
M124L	smooth muscle myosin light chain kinase	partial (InfoCard)	81 % (InfoCard)
wildtype from Bos taurus		yes (InfoCard)	100 % (InfoCard)	1.5 (InfoCard)

M124L	calmodulin-dependent cyclic nucleotide phosphodiesterase	yes (InfoCard)

M124L	neuronal nitric oxide synthase	partial (InfoCard)	65 % (InfoCard)