The information presented here was taken from Nakayama,S., N. Moncrief, and R. Kretsinger, (1992) J. Mol. Evol. 34, 416-448. However, any inaccuracies are my own.

The following proteins were found to be congruent, meaning that their individual domains branch together: calmodulin, troponin C, essential light chain of myosin, regulatory light chain of myosin, call, squidulin, CDC31, caltractin, and calcium-dependent protein kinase.

General Consequences of this Scheme

Branch Points of Specific Protein

Because these proteins are congruent with each other, all four of their EF hands can be used simultaneously to derive their evolutionary relationship, as shown below:

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