AMBER Archive (2009)

Subject: Re: [AMBER] How to denature DNA

From: Adrian Roitberg (roitberg_at_qtp.ufl.edu)
Date: Tue Sep 08 2009 - 09:04:30 CDT


Dear balaji,

You are confusing a number of issues here.
DNA can denature by a number of methods, 99% of which does not involve
pulling anything.
The usual methods involve raising the temperature, adding chemical
denaturants, etc.

Pulling DNA strands is a state of the art experimental technique, that
probably has little to do with denaturation.

Adrian

balaji nagarajan wrote:
> Dear Amber !
>
> I would like to denature the DNA,
> is it possible to pull the DNA on both the strands
> so that one can study how it denature.
>
> regards
> balaji
>
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-- 
                            Dr. Adrian E. Roitberg
                              Associate Professor
                             Quantum Theory Project
                            Department of Chemistry

Senior Editor. Journal of Physical Chemistry American Chemical Society

University of Florida PHONE 352 392-6972 P.O. Box 118435 FAX 352 392-8722 Gainesville, FL 32611-8435 Email adrian_at_qtp.ufl.edu

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