Chazin Home Chazin Home | Ca-binding Protein DB | Vanderbilt Home Vanderbilt Home
Processing 1D data with felix | Publications | Wisdom | Search

How to process a 1D bruker data file in felix

per Shibani:

  1. open new dba
  2. open fid file
  3. look in aqus and find DECIM (decimation) and DSPFVS values (e.g. 24, 12) These will vary depending on spectral width
  4. def datype 1 (tell it we're working with complex data)
  5. def datsize 4096 (this is also in acqus, under __)
  6. zoom on beginning, note that the fid starts after about 70 points
  7. csl 70 (circular shift left - puts first points at the end of your fid)
  8. dr (drive - activates all those commands, redraws window)
  9. def datsize 4026 (apply the following commands without the last 70 points)
  10. dr
  11. cnv 1 30 (watersuppression: convolute function, modeflag, windowfunction size)
  12. dr
  13. ss 2048 60 (window function: sinesquarebell, phase shift)
  14. dr
  15. def datsize 4096 (now we re-include the excluded points)
  16. csr 70 (bring those points back to the front)
  17. dft 24 12 (digital fourier transform, using parameters __ __)
  18. dr
  19. phase correction (interactive)
  20. baseline (interactive; polynomial = 2)
  21. reference (using water, set center of spectrum 4096 to 4.76ppm)
Back to my home page
created 6-12-01 by Jonathan.SheehanATvanderbilt.edu