pSV281 dna sequence 
clone into 5' BamHI site

cgatcccgcgaaattaatacgactcactataggggaattgtgagcggata
acaattcccctctagaaataattttgtttaactttaagaaggagatataC
ATATGggttcttctcaccatcaccatcaccatgaaaacctgtacttccag
GGATCCccGAATTCgagctccgtcgacaagcttgcggccgcactcgagat
caaacgggctagccagccagaactcgccccggaagaccccgaggatgtcg
agcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaag
gaagctgagttggctgctgccaccgctgagcaataactagcataacccct
tggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaacta
tatccggattggcgaatgggacgcgccctgtagcggcgcattaagcgcgg
cgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgcccta
gcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccgg
ctttccccgtcaagctctaaatcgggggctccctttagggttccgattta
gtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttca
cgtagtgggccatcgccctgatagacggtttttcgccctttgacgttgga
gtccacgttctttaatagtggactcttgttccaaactggaacaacactca
accctatctcggtctattcttttgatttataagggattttgccgatttcg
gcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattt
taacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatg
tgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtat
ccgctcatgaattaattcttagaaaaactcatcgagcatcaaatgaaact
gcaatttattcatatcaggattatcaataccatatttttgaaaaagccgt
ttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaag
atcctggtatcggtctgcgattccgactcgtccaacatcaatacaaccta
ttaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatga
gtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttcca
gacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcat
caaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacg
cgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcg
caggaacactgccagcgcatcaacaatattttcacctgaatcaggatatt
cttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaac
catgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcat
aaattccgtcagccagtttagtctgaccatctcatctgtaacatcattgg
caacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttc
ccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagc
ccatttatacccatataaatcagcatccatgttggaatttaatcgcggcc
tagagcaagacgtttcccgttgaatatggctcataacaccccttgtatta
ctgtttatgtaagcagacagttttattgttcatgaccaaaatcccttaac
gtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaagga
tcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaa
aaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaa
ctctttttccgaaggtaactggcttcagcagagcgcagataccaaatact
gtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagc
accgcctacatacctcgctctgctaatcctgttaccagtggctgctgcca
gtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccg
gataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccag
cttggagcgaacgacctacaccgaactgagatacctacagcgtgagctat
gagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggta
agcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaa
cgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagc
gtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgcc
agcaacgcggcctttttacggttcctggccttttgctggccttttgctca
catgttctttcctgcgttatcccctgattctgtggataaccgtattaccg
cctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagc
gagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctcct
tacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtac
aatctgctctgatgccgcatagttaagccagtatacactccgctatcgct
acgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacg
cgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtg
accgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaa
acgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgatt
cacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccaga
agcgttaatgtctggcttctgataaagcgggccatgttaagggcggtttt
ttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgg
gggtaatgataccgatgaaacgagagaggatgctcacgatacgggttact
gatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggc
ggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagc
gcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcct
gcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttc
cagactttacgaaacacggaaaccgaagaccattcatgttgttgctcagg
tcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggt
gattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcct
caacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcga
taatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaag
gcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgat
catcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcg
ctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagt
gcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggtt
gaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagct
aacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaac
ctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcgg
tttgcgtattgggcgcAagggtggtttttcttttcaccagtgagacgggc
aacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcg
gtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggtta
acggcgggatataacatgagctgtcttcggtatcgtcgtatcccactacc
gagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgc
gcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgc
cctcattcagcatttgcatggtttgttgaaaaccggacatggcactccag
tcgccttcccgttccgctatcggctgaatttgattgcgagtgagatattt
atgccagccagccagacgcagacgcgccgagacagaacttaatgggcccg
ctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgccc
agtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctg
gtcagagacatcaagaaataacgccggaacattagtgcaggcagcttcca
cagcaatggcatcctggtcatccagcggatagttaatgatcagcccactg
acgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgcc
gcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgc
gagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactg
gaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgc
cacgcggttgggaatgtaattcagctccgccatcgccgcttccacttttt
cccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacg
gtctgataagagacaccggcatactctgcgacatcgtataacgttactgg
tttcacattcaccaccctgaattgactctcttccgggcgctatcatgcca
taccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctc
tcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggc
cgttgagcaccgccgccgcaaggaatggtGCATGCgcaacgccaatcagc
aacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaatt
cagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgt
ggctggcctggttcaccacgcgggaaacggtctgataagagacaccggca
tactctgcgacatcgtataacgttactggtttcacAGATCT